烟草内生细菌YN201448的定殖能力研究.pdf

80 Chinese Tobacco Science 2015-0636 3 YN201448 1 1,3 2,3 2,3 1,2* 1. 650201 2. 650201 3. 650471 YN201448 pGFP4412 YN201448 GFP 48-pGFP48-pGFP GFP 5 YN201448 48-pGFP ﹥﹥ YN201448 S435.72 1007-5119201503-0080-06 DOI 10.13496/j.issn.1007-5119.2015.03.016 The Colonization of Endophytic Bacterium YN201448 in Tobacco 1 1,3 2,3 2,3 1,2* YANG Zhenfu , HE Pengfei , WU Yixin , MAO Zichao , HE Yueqiu (1. Faculty of Plant Protection, Yunnan Agricultural University (YAU), Kunming 650201, China; 2. Faculty of Agronomy and Biotechnology, YAU, Kunming 650201, China; 3. National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains, Kunming 650471, China) Abstract: In order to determine the colonization of endophytic bacterium YN201448 in tobacco, we successfully obtained a stable GFP tagged strain 48-pGFP by transferring the pGFP4412 plasmid into YN201448 via natural transformation. 48-pGFP showed the same antagonistic effect against 5 pathogenic fungi as the wild strain YN201448. 48-pGFP was observed to be distributed in root, stem and leaf tissues of tobacco after its seeds soaked and seedling roots drenched with 48-pGFP. The colonizing bacterium decreased with the trend of root stem leaf, and 48-pGFP could colonize in rhizospheric soil stably. Fluorescence microscope observation indicated that 48-pGFP mainly appeared in tobacco stem epidermis, cortex and vascular tissue cells. Therefore, YN201448 could colonize inside and around tobacco plant stably for a relatively long period of time. Keywords: Bacillus amyloliquefaciens ; GFP; antagonistic effect; natural transformation [6]