分子生物学考题–林富邦老师部份.DOCVIP

分子生物學 考題 – 林富邦老師部份 一 單選題 (每題2分 共18分) 1. When the cellular level of tryptophan decreases in E. coli: A. tryptophan-bound Trp repressor associates with trp operator. B. Trp aporepressor has lowered affinity for trp promoter allowing RNA. polymerase binding and transcription of the trp operon. C. tryptophan binds trp inducer to promote positive control of trp promoter. D. trp repressor has a greater affinity for trp operator. E. none of the above. 2. How many high-energy phosphoric anhydride bonds per amino acid for the energy expenditure for protein synthesis A. 0 B. 1 C. 2 D. 3 E. 3. Termination of translation in prokaryotic cells requires: binding of the terminator tRNA to the termination codon. interaction of release factors with the termination codon. ternary interaction of the release factor and the termination tRNA with the termination codon. release factor interaction with the Shine-Dalgarno sequence and subsequent dissociation of the two ribosomal subunits. displacement of EF-G by EF-Tu:aminoacyl-tRNA 4. Eukaryotic secretory proteins are synthesized and translocated via the endoplasmic reticulum. Order the following sequence of events for this process. A. signal sequence removed. B. glycosylation in the ER lumen. C. signal sequence synthesis on ribosomes. D. SRP binds signal sequence and subsequently binds SRP-receptor. E. ribosome dissociates. A. A, C, E, B, D B. C, D, A, B, E C. C, A, D, B, E D. A, C, B, D, E E. C, B, D, E, A 5. Translation of a synthetic polyribonucleotide containing the repeating sequence CAA in a cell-free protein-synthesizing system produced three homopolypeptides: polyglutamine, polyasparagine, and polythreonine. If the codons for glutamine and asparagine are CAA and AAC, respectively, which of the following triplets is a codon for threonine? ACC B. CAA C. CAC D. CCA E. ACA 6. Cyclic AMP regulates the lac operon by binding to the operator to turn on transcription binding to the lac repressor to