细胞膜表面糖识别芯片的制备及其初步应用.pdf

中国医科大学学报 第37卷 第5期 2008年10月 577 JournalofChinaMedicalUniversity Vol.37 No.5 Oct.2008 · · 细胞膜表面糖识别芯片的制备及其初步应用 1 1 2 1 1 1 1 1 何群 ，宋春阳，张艳，潘忠诚，王天骄，房凯，张玉魁，赵雨杰 （1.中国医科大学 生物芯片中心，教育部细胞生物学重点实验室，沈阳110001；2.沈阳医学院 细胞生物学教研室，沈阳110034） 摘要：目的 制备可以对细胞膜表面糖链进行识别的凝集素芯片并进行初步应用。方法 在芯片上制备22 种凝集素点阵，提 取组织和体液细胞进行荧光标记，利用凝集素与细胞膜表面糖链特异亲和性对细胞进行自动捕获，激光扫描仪和光镜检测。结 果 根据芯片上各个凝集素位点捕获细胞情况和该点凝集素的特异性获得细胞膜表面糖谱，并在光镜下对细胞数量形态进行观 察。芯片的重复性和稳定性较好。结论 该凝集素芯片可以对细胞进行自动捕获，对细胞膜表面糖链进行总体、即时的观察，对细 胞膜糖链特征有一个总体的认识，建立各种细胞膜表面糖谱，为细胞膜表面糖链特征检测提供一个新的分析方法。 关键词：凝集素；糖组学；细胞膜 中图分类号：R392.12 文献标志码：A 文章编号：0258-4646 （2008）05-0577-03 Preparation of Lectin Microarray and Cell Surface Glycome Profiling 1 1 2 1 1 1 1 HE Qun ，SONG Chun-yang ，ZHANG Yan ，PAN Zhong-cheng ，WANG Tian-jiao ，FANG Kai ，ZHANG Yu-kui ， ZHAO Yu-jie1 （1. Biochip Center，Key Labotory of Ministry of Education，China Medical University，Shenyang 110001，China ；2. Department of Cytobiology，Shenyang Medical College，Shenyang 110034，China） Abstract ：Objective To develop a lectin microarray which can analyze the cell-surface glycome. Methods 22 lectins were printed on array，and the cells extracted from tissue and blood were labeled with fluorescence and caught by lectins through the distinctive binding specificities，and bound cells were directly detected by the laser fluorescence scanner or were observed under microscope after staining. Re－ sults Glycan profiling of cell membrane was obtained by distinctive binding specificities of lectins on microarray and the appearance of bound cells were observed under the microscope. The lectin micrearray was very stable and result was reproducible. Conclusion The lectin micr