肝郁脾虚证模型大鼠下丘脑.pdfVIP

Progress in Modern Biomedicine 2006 Vol. 6 No. 4 10 肝郁脾虚证模型大鼠下丘脑- 垂体- 肾上腺皮质的变化 李艳彦 谢 鸣 陈 禹 王洪海 ( 北京 100029) : 探讨复合造模法复制肝郁脾虚证大鼠模型的HPA 轴变 及特点: 大鼠采用慢性束缚+ 过度疲劳+ 饮食 失节法, 连续三周, 自然恢复一周观察造模期间各不同时间点大鼠肾上腺重量及指数下丘脑促肾上腺皮质激素释放激素 ( CRH) 血浆促肾上腺皮质激素(ACTH) 及皮质酮( CORT) 的变 : 与正常组比较, 模型大鼠肾上腺重量第一周显著增加(P 0.001) , 第三四周显著减低(P 0.001和P 0.01) , 其肾上腺指数在实验各周均显著高于正常组(P 0.05 和P 0.01) ; 下丘脑 CRH 第三周显著低于正常组(P 0.05) , 第四周回升并显著高于正常组(P 0.05) ; 血浆ACTH 在第一三四周均显著降低(P 0. 05) ; 血浆CORT 第一周显著降低(P 0.05) , 第二周有所回复, 第三四周仍显著降低(P 0.05 和P 0. 001) : 该肝郁脾虚证 大鼠模型存在HPA 轴的功能减低, 垂体对上位激素调节的反应能力减低可能是该模型的一个重要的生物学特征 : 肝郁脾虚证; 动物模型; 大鼠; HPA 轴; 复合造模法 Study on Change of Hypothalamus- pituitary- adrenal Axis(HPAA) in TCMs Syndrome Model of YPX LI Yan- yan, XIE Ming, CHEN Yu, WANG Hong- hai ( Beij ing University of Chinese Me icine, Beji ing 100029, China) ABSTRACT Objective: To study the cha ge of Hypothalamus- pituitary- adre al axis( HPAA) i the TCM s sy drome model of GYPX( stag atio of liver- qi a d deficie cy of splee - qi) made by a ew compou d method. Methods: Chro ic astricti gexcess fatigue a d out of co sta t diet were adopted towards the rats for three weeks ru i g a d the the rats were restored aturally for o e week. The cha ges of the weight a d i dex of rats adre al gla d a d the levels of Corticotropi - releasi g Hormo e( CRH) i hypothalamus, Adre ocorticotropicHor- mo e(ACTH) a d Corticotrophi ( CORT) i plasmawere observed at differe t time duri g maki g model. Results: Comparedwith releva t or- mal group, the weight of the model rats adre al gla d i creased sig ifica tly at the 1st week(P 0.001) a d decreased at the 3rd a d 4th week ( P 0.001 a d P 0.01) , but the i dex of adre al gla d i crease