西华大学毕业样本生物工程学院.docVIP

TOC \o 1-5 \h \z 目 录 摘要 1 HYPERLINK \l bookmark4 \o Current Document \h Abstract 1 1前言 2 1.1原生质体融合技术的发展 2 HYPERLINK \l bookmark6 \o Current Document \h 2材料与方法 3 2.1材料与仪器 3 2.2实验方法 3 HYPERLINK \l bookmark8 \o Current Document \h 3实验结果与分析 4 3.1各种灭活方法的最佳灭活条件 4 HYPERLINK \l bookmark10 \o Current Document \h 4结论 4 4.1灭活方法的结论 4 HYPERLINK \l bookmark12 \o Current Document \h 总结与体会 4 HYPERLINK \l bookmark14 \o Current Document \h 致谢词 4 参考文献 4 摘 要 本实验对米曲霉 Aspergillums 平处3.951 和 Asp纣gillus oryzae CICC2339的原 生质体分别进行紫外线、微波和超声波灭活，探讨各种灭活方法的最佳灭活条件。 然后在各种灭活方法的最佳条件下对双亲原生质体进行灭活，用PEG作融合剂进 行原生质体融合。各从融合子中选出8株生长速度快、菌落直径大的融合株，接种 到酪素平板上测其He值，并与亲本菌株进行比较。同时比较各种灭活方式的融合 率及He值，从中找出最大He值的灭活方式。结果发现，亲本菌株Aspergillus oryzae CICC2339采用微波灭活方法，Aspergillus oryzae 3.951采用微波灭活方法时He值 最大，也就是该种灭活方式是最佳的灭活方式，从而筛选出8株菌株，这8株融合 株的He值均比生长速度快而产酶量低的亲本菌株Aspergillus oryzaeAS3.951大， 且均超过了生长速度慢但产酶量大的亲本菌株Aspergillus oryzae CICC2339。 【关键词】米曲霉；原生质体；灭活；融合 Abstract This experiment inactivated protoplast of Aspergillus oryzae 3.951 which growmuch faster and Aspergillus oryzae CICC2339 which produce much better enzyme with ultraviolets microwave and ultrasonic separately. Though this we could find the best term of each method for inactivating.After that we use these methods to inactivate the two kinds protoplasts separately. Then we used PEG to fuse inactivated protoplasts. Screened 8 individual plants which grown faster and the diameter was bigger in each method of fusion, inoculated them to the flat of casein, measured the value of He, then compared with the original strains. At the same time ,we compare the average He value and find the highest He value? The result indicated that when inactivate the Aspergillus oryzd?CICC2339 with microwave and inactivate Aspergillus oryzae 3.951 with ultraviolet is the best method.So we select 8 strains whose He value were higher than the original strain Aspergillus oryzae 3.951 and the value of these strains even higher than the original strain Aspergillus oryzae CICC2339. Keywords: