油菜蜂花粉黄酮醇苷的体内外抗氧化研究-食品科学.PDF

※营养卫生 食品科学 2010, Vol. 31, No. 19 359 1 1 2 2 1 孙丽萍 ，徐 响 ，廖 磊 ，刘魁英 ，杨佳林 (1. 中国农业科学院蜜蜂研究所，北京 100093 ； 2.北京市卫生局临床药学研究所，北京 100035) ：以油菜蜂花粉黄酮醇苷组分为原料，以还原力和DPPH 自由基清除率为指标研究黄酮醇苷在化学模拟体 系中的抗氧化活性。结果表明，油菜蜂花粉黄酮醇苷的抗氧化活性不及其苷元成分。以 CCl4 制备小鼠脂质过氧化 损伤模型，通过体内自由基发生体系进一步研究花粉黄酮醇苷的抗氧化作用，并对可能的作用机制进行探讨。结 果显示：油菜蜂花粉黄酮醇苷组分能够显著地降低脂质过氧化小鼠肝匀浆和血清中 MDA 含量，提高小鼠 SOD 活 性和总抗氧化能力(T-AOC)，对脂质过氧化损伤有很好的保护作用，原因可能是体内有关酶系可将黄酮醇苷水解使 其游离出酚羟基而发挥作用。本研究进一步肯定了油菜蜂花粉黄酮醇苷对机体氧化损伤的保护作用。 ：油菜蜂花粉；黄酮醇苷；抗氧化活性；脂质过氧化 Anti-oxidant Effect of Flavonoid Glycosides from Rape Bee Pollen 1 1 2 2 1 SUN Li-ping ，XU Xiang ，LIAO Lei ，LIU Kui-ying ，YANG Jia-lin (1. Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China； 2. Beijing Clinical Pharmacy Institute, Beijing 100035, China) Abstract ：Flavonoid glycosides were prepared from rape bee pollen, and their ability to scavenge DPPH free radicals and reducing power in simulated chemical systems were determined. The antioxidant activity of the extracted flavonoid glycosides was not as strong as that of kaempferol or quercetin. The antioxidant activity of the extracted compounds in vivo against CCl4-induced lipid peroxidation in mice was investigated, and the antioxidant mechanisms were also elucidated. The results showed that the extract could significantly inhibit the generation of MDA in liver, and increase the level of T-AOC in liver and serum. The possible antioxidant mechanism was the generation of flavonoid aglycone with plentiful antioxidant phenolic groups due to the hydroly- sis of flavonoid glycosides by in vivo