添加无机氮磷施肥法对养殖海水鱼苗之嗜食饵料生物及胃内容物调查.DOC

添加無機氮、磷施肥法對養殖海水魚苗之嗜食 餌料生物及胃內容物調查 Effects of inorganic phosphorus and nitrogen fertilization on feeding preferences and stomach contents of marine fish larviculture 研究生：陳建華 Chen, Chien-Hua 指導教授：翁韶蓮 Wong, Saou-Lien 張桂祥 Tew, Kwee Siong 呂明毅 Leu, Ming-Yih 【摘要】 海水魚苗養殖上餌料適口性是最大的問題。本研究於實驗組 (F) 中添加氮鹽在700 μg/l、磷鹽在100 μg/l，使浮游生物自然產生，以探討魚苗之初期餌料上之選擇及嗜食性，而控制組 (N) 則不添加營養鹽只投餵輪蟲 (Brachionus rotundiformis)，並比較魚苗成長與活存率。結果顯示F組的赤鰭笛鯛 (Lutjanus erythropterus) 在孵化第三天 (3 DPH, days post hatch) ，魚苗攝食俠盜蟲屬纖毛蟲 (Strobilidium sp.) ，N組則有攝食輪蟲；在21天後N組魚苗活存率為2.2 ± 0.8 % 顯著高於F組為0 % (p0.05) 。在鞍帶石斑魚 (Epinephelus lanceolatus) 養殖實驗中，F組魚苗在孵化第3 – 5天攝食纖毛蟲 (3.59 ± 0.47 ind/fish) ，孵化8 – 24天攝食橈足類無節幼生，實驗結束時活存率為0.3 %；N組則在第八天全部死亡。點帶石斑魚 (Epinephelus coioides) 養殖實驗中，F組魚苗在開口後攝食渦鞭毛藻，緊接著攝食輪蟲，而N組魚苗開口後即直接攝食輪蟲，兩組的活存率以N組 (8.1 ± 2.1 %) 高於F組 (2.3 ± 0.8 %) ，但無顯著性差異 (p0.05) 。赤鰭笛鯛與點帶石斑魚的開口口徑 (221 ± 21 μm、181 ± 12 μm) 大於鞍帶石斑魚開口 (157 ± 5 μm) ，可直接攝食SS型輪蟲，鞍帶石斑則必須攝食更小的餌料生物 (如纖毛蟲) 才能活存。本實驗顯示添加營養鹽方法可提供較小型的餌料生物，有助於海水魚苗初期攝食。 關鍵字：無機營養鹽，餌料嗜好，赤鰭笛鯛，鞍帶石斑魚，點帶石斑魚，種苗培育 【Abstract】 Marine fish larviculture has been unsuccessful partly due to the lack of suitable size of food. In this study, we maintained inorganic nitrogen at 700 μg/l and phosphorus at 100 μg/l to induce growth of different sizes of plankton in experimental group (F group), and no addition of nutrient in controlled group (N group) to compare the growth and survival rates. The results showed that the red snapper (Lutjanus erythropterus) were raised in F group, they started feeding on oligotrich ciliates (Strobilidium sulcatum) at 3 days post hatch (DPH), while those in the N group fed on rotifers (Brachionus rotundiformis) that we added. The survival rate in N group (2.2 ± 0.8 %) was significantly higher (p0.05) than in F group (0 %). When giant grouper (Epinephelus lanceolatus) was raised, in F group they fed on ciliates at 3 – 5 DPH (3.59 ± 0.47 ind/fish), and on copepods nauplii at 8 – 24 DPH, while those fed with rotifer in N group died off after 5