气相色谱-质谱联用法测定烟草及烟草制品中的麦角甾醇-烟草科技.PDF

烟草科技/TobaccoScienceTechnology 烟草化学/Tobacco Chemistry 2013年第5期（总第310期） 气相色谱-质谱联用法测定烟草及烟草制品中的麦角甾醇 * 戚大伟，林华清 ，沙云菲，吴 达，刘百战 上海烟草集团有限责任公司技术中心，上海市杨浦区长阳路717号 200082 摘要：为准确测定烟草及烟草制品中麦角甾醇的含量，建立了以7-去氢胆固醇为内标，将麦角甾醇和7- 去氢胆固醇衍生后进行GC/MS 同步选择离子检测/ 全扫描的定量检测方法，并采用该法测定了不同种类 2 烟叶及卷烟中的麦角甾醇含量。结果表明：该方法在2.0 ～200.0 μg/mL 之间具有良好的线性关系，R= 0.9995 。方法的检出限和检测限分别为0.46 和1.53 mg/kg ，回收率在85.6% ～91.3% 之间。该方法适用于 烟草及烟草制品中麦角甾醇的定量检测。 关键词：烟草；烟草制品；霉菌污染标志物；麦角甾醇；7-去氢胆固醇；GC/MS 中图分类号：TS411 文献标识码：A 文章编号：1002-0861 （2013 ）05-0041-05 Determination of Ergosterol in Tobacco and Tobacco Products with Gas Chromatography/Mass Spectrometry * QI Dawei ，LIN Huaqing ，SHA Yunfei ，WU Da ，and LIU Baizhan Technology Center ，Shanghai Tobacco Group Co. ，Ltd. ，Shanghai 200082 ，China Abstract: In order to accurately determine the ergosterol in tobacco and tobacco products ，a quantitative determination method by GC/MS with selected ion monitoring/full scan mode was established by taking 7-dehydrocholesterol as an internal standard and derivatizing before GC/MS analysis ，and the contents of ergosterol in tobacco leaves and cigarettes of different kinds were determined. The results showed that the calibration curve exhibited good linearity with a correlation coefficient of 0.9995 in the range of 2.0 to 200.0 µg/mL. The LOD and LOQ were 0.46 and 1.53 mg/kg ，respectively. The recovery ranged from 85.6% to 91.3%. This method is suitable for the quantitative determination of ergosterol in tobacco and tobacco products. Keywords: Tobacco ；Tobacco product ；Marker of mold infection ；Ergosterol ；7-Dehydrocholesterol ；GC/MS 烟草及烟草制品（烟丝）中存在的真菌菌丝和孢子， 此外，麦角甾醇也是卷烟烟气中气态烷烃和稠环芳烃的 在适宜的环境条件下会大量繁殖而发生霉变，这会带来 重要来源之一。据文献报道，随着麦角甾醇含量增加，卷 不必要的经济损失。因此，烟草及烟草制品的霉变控制