青蒿琥酯在体外对胰腺癌Panc-1细胞抑制和杀伤-第三军医大学学报.DOCVIP

青蒿琥酯在体外对胰腺癌Panc-1细胞抑制和杀伤作用的初步研究 谢宏民，杨新魁，李佳璇 （512415 广州，广东医学院附属陈星海医院消化内科） [摘要] 目的 探讨青蒿琥酯（artesunate，ART）在体外对胰腺癌Panc-1细胞抑制和杀伤作用。方法 采用台盼蓝排斥实验再次证实ART对胰腺癌Panc-1细胞增殖抑制及杀伤作用，流式细胞技术检测细胞周期变化及凋亡率。结果结果应和方法相对应。注意表述时的归纳总结，并突出实验组的结果；要把做过统计学分析得出主要结论的数据和统计学结果全部写入，请添加，英文也需做相应的修改；已修改. 台盼蓝排斥实验表明随着ART浓度的增加，作用时间的延长，Panc-1细胞细胞数量和存活率明显降低(P值小于0.05)，而在同等条件下对人正常肝细胞HL-7702则无明显抑制作用。PI染色流式细胞仪检测结果提示ART将Panc-1细胞阻滞在G2 /M期，但无凋亡峰出现。结论 证实ART在体外对胰腺癌Panc-1细胞有增殖抑制及杀伤作用，将细胞周期阻滞在G2/M期。 结果应和方法相对应。注意表述时的归纳总结，并突出实验组的结果；要把做过统计学分析得出主要结论的数据和统计学结果全部写入，请添加，英文也需做相应的修改；已修改. [关键词] 青蒿琥酯；胰腺癌；凋亡 [中图法分类号] [文献标志码] A The preliminary study of the growth inhibition and anti-personnel role of ART in vitro of pancreatic cancer Xie Hongmin,Yang Xinkui,Li Jiaxuan() [Abstract] Objective Artesunate (artesunate, ART) is extracted from Artemisia annua effective anti-malarial composition, can inhibit a variety of tumor cell proliferation and induced apoptosis of tumor cells, but the mechanism is not yet completely clear. This study was designed to explore ART in vitro of pancreatic cancer have growth inhibition and anti-personnel role, for artesunate inhibited pancreatic provide adequate theoretical basis for the treatment of pancreatic cancer to explore new strategies for thinking. Methods Using trypan blue exclusion test observate ART of pancreatic cancer Panc-1 cell growth inhibition and killing effect, flow cytometry（FCM） detection of cell cycle and apoptosis rate。Results Trypan blue exclusion test showed that the increase in concentration and time of the ART, Panc-1 cell survival rate significantly decreased, while under the same conditions of human normal liver cells, HL-7702 had no significant inhibitory effect. PI staining flow cytometry detection results suggest that ART will block the cell cycle at the G2 / M phase, at the same time did not appear apoptotic peak. Conclusions ART in vitro of pancreatic cancer Panc-1 cells have Growth inhibition and anti-personnel ro