基于COI基因序列的中国东南沿海四指马鲅属鱼类的遗传多样性分析-微生物学专业论文.docxVIP

暨南大学硕士学位论文 暨南大学硕士学位论文 PAGE PAGE VI Abstract Eleutheronema fishes are valuable marine fish widely distributed in Indo-West Pacific, they are also important aquacultural objects. Based on external morphological characteristics 194 specimens from 9 geographical populations , Partial mitochondrial cytochrome oxidase subunit 1 of some samples were sequenced via PCR amplification and DNA direct sequencing mothed. Combined with GenBank available homologous sequences from Indian Ocean, northern coast of Australia, Xisha Islands and Nansha Islands from China, altogether 207 Eleutheronema mtDNA COI sequences were analyzed to infer the genetic diversity and population structure. The results were as following: The species status of studied Eleutheronema Three significant genealogical branches were recognized on the neighbor-joining tree of the Eleutheronema fishes. The results of genetic distances, genetic differentiation index, external morphological characteristics of the Eleutheronema and the historical material confirmed the species status. Most Eleutheronema fish in the East and South China Seas is E. rhadinum. Some specimens distributed in Dongxing and Fuzhou,China, and all of the specimens in the north of Australiashoud be treated as a valid species E. coecus Macleay. In addition, there are larger genetic differentiation between groups from the coast of China and those from the coast of northern Australian, indicating that there might exist a trend from them to become different species. 1 specimens from Dongxing,China and all Indian Ocean specimens are E.tetradactylum according to the information of typotype.However, taking it into consideration that it is difficult to rule out the possibility of the existence of additional genealogical branches in this the vast water area from Indian Ocean to the coastal of Dongxing ,China .So here it is treated as E. cf. tetradactylum Temporarily. The genetic diversity and the genetic differentiation of Eleutheronema The haplotype d