健康人肠道大肠埃希菌耐药性与Ⅰ类整合子关系的研究-营养与食品卫生学专业论文.docxVIP

四川大学硕士学位论文Study 四川大学硕士学位论文 Study on the Relationship between Resistance ofEscherichia coli in Healthy Human and Class I Integrons Postgraduate：Fang Mei Supervisor：．加socinte ProfessorL蛔Hengchuan 0bjective To investigate the level of antibiotic resistance,the frequency of class I inIc_则in Escherichia∞站isolated from healthy human in Shenzhen,and analyze the relationship between resistance genes carded by class I integrons and antibiotic resistance phenotypes．To observe the role of class I integrons in the acquisition and transmission of antibiotic resistance among Gram-negative badllj．To study the molecular mechanism of integrons and gene casscHes in mediation and transmission of antibiotic resistance．The study laid a theoretical foundation for the control of antibiotic resistance and multi-drug resistant bacteria,the reasonable usage of antibiotics in clinical treaUnenL Mthods Escherichia coil isolated from healthy human WCrC identified． Susceptibility of 150 Escherichia coli islates to 16 different antibiotics was determined by the disk diffusion method．Analyze the plasmid profile of isolates and the resistance strailLs were classified．The presence of class I integrase(intll)in the isolates were detected by PCR amplifing total DNA． Class I integrons of intll positive strains were detected by PCR amplifing total DNA and plasmid DNA respectively．The amplicons of class I衄egr0璐with identical length were analyzed by RFLP and class I integr0璐with different restriction patterns were sequenced．To observe the gene cas∞flcs and its· arrays in class I integmns by searching the sequences in Genbank．Gene 3 四川大学硕士学位论文localization 四川大学硕士学位论文 localization of class I integrons was finished by the test of plasmid elimination． Study on the horizontal transfer of dass l integrons among Escherichia∞雎 was carried with plasmid conjugation experiments． ResuIts The results of susceptibility test showed that over 35％of tested stra泌 were resistant to 6 antibioties．They weTe Teracycline(63．33