健脾渗湿法治疗特应性皮炎的疗效及对患者免疫功能调节作用的研究-中医外科学专业论文.docxVIP

表Th2型细胞因子，以白介素12代表Thl型细胞因子)、RT—PCR法检测外周血PBMC中 表Th2型细胞因子，以白介素12代表Thl型细胞因子)、RT—PCR法检测外周血PBMC中 GATA一3基因的表达状况，发现治疗前AD患者血清IgE水平显著高于正常对照组 (PO．01)，血清中IL一4、10水平显著高于正常对照组(PO．01)，而IL一12水平低 于正常对照组(PO．05)，外周血PBMC中GATA一3mRNA水平显著高于正常对照组 (P0．05)，因此，除血清IgE升高、Th2／Thl型细胞因子平衡失调的经典改变外，转 录因子GATA一3的高表达是特应性皮炎的另一个重要改变，并且相关分析得出IL一4、lO 均与GATA一3呈显著正相关，说明转录因子GATA-3基因的过度表达可能是导致AD Th2型 细胞因子明显升高的重要原因，因此抑制转录因子GATA-3的高表达可能是今后药物治 疗AD可选择的一个新的策略和靶位。 3．特应性皮炎方可抑制外周血PBMC中转录因子GATA-3基因的高表达、降低Th2型细胞 因子来调节Th2／Thl平衡。特应性皮炎方治疗AD的有效的机制可能部分与此相关。 4．特应性皮炎方是导师榻国维教授在健脾渗湿法的指导下组成，用于治疗AD的经验 方，课题中，无论是对临床症状的改善，还是对患者免疫功能的调节，都客观的说明 了该方的有效性，也验证了健脾渗湿法治疗AD这一理论的正确性。 关键词特应性皮炎；细胞因子；GATA-3；基因表达；中药 The The CI inicaI effect of strengthening spIeen and ext racti ng water damp i n treati ng atopiC dermatiti S and its reguIation in the immune funotion of the patients Abstract SpeciaI ity：DermatoIogyVenereoIogy of TOM Author：Wang Xin Tutor：Prof．Xuan Guo-we i Object iyes First to investigate the mechanism of Atopic Dermatitis(AD)more deeply through measuring IgE、Th2／Thl Types Cytokines in serum and the expression of TranscriDtion factor GATA一3mRNA in PBMC of the patients with AD．Then to investigate the clinical effect of ADC(Atopic Dermatitis Compound)in treating AD，and to find its possible mechanism to direct our cl inical practice and to make basis of the development of new Traditional Chinese medicine Methods In the clinical part．select 28 AD patients with symptoms of spleen—deficiency and retention of dampness to be the object，and then give them ADC to eat for three months．recording their SCORAD before and after the treatment one month and three months respectively． In the exprimental part，pick 15 out of the 28 patients with AD randomly and select 15 healthy controls，then apply ELISA technique to detect their IgE、Th2／Thl Types Cytokines in serum and RT—PCR technique to detect the expression of Transcription factor GATA一3mRNA in PBMC respectively． Results After treated by ADC for three months，the SCORAD of the 28 patients w