一株甲烷氧化菌的分离鉴定与特性.PDFVIP

微生物学通报 SEP 20, 2010, 37(9): 1265−1271 Microbiology China © 2010 by Institute of Microbiology, CAS tongbao@ 研究报告 一株甲烷氧化菌的分离鉴定与特性 刘晓宁 李珍 林国秀 王培培 王立明 张杰* ( 四川大学生命科学学院 资源微生物与微生物技术四川省重点实验室 四川 成都 610064) 摘 要: 甲烷氧化菌是一类能以甲烷作为唯一碳源和能源进行同化和异化代谢的微生物。从若尔盖 高原不同地点采集的样品中筛选得到一株名为XN1 的甲烷氧化菌, 根据此菌株的形态与 16S rRNA 序列同源性分析, 证实该菌株属于Methylomonas 属。对该菌株的培养条件进行研究的结果表明, 以 甲烷与甲醇共同作为碳源, 硝酸钾和氯化铵共同为氮源时菌生长最好, 最适生长温度为25°C, 最适生 长pH 为6.5, 培养基中CuSO ·5H O 和FeSO ·7H O 的浓度以0.03 mg/L 和0.4 mg/L 为宜。 4 2 4 2 关键词: 甲烷氧化菌, 分离鉴定, 特征, 培养条件 Isolation, Identification and Characterization of Methanotrophic Bacteria LIU Xiao-Ning LI Zhen LIN Guo-Xiu WANG Pei-Pei WANG Li-Ming * ZHANG Jie (Key Laboratory of Microbial-resources and Microbial-technology, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, China) Abstract: Methanotrophs are a kind of bacteria which can use methane as the sole carbon source and energy for their anabolism and catabolism. A strain methanotroph was isolated from different samples of the Zoige Plateau, named XN1, and was studied. It was identified a strain of Methylomonas through 16S rRNA sequencing and comparison. The investigation result showed that the optimal conditions for the strain were methane gas together with methanol as carbon source and the coexistence