甲基乙二醛对胰腺癌PANC-1细胞增殖及凋亡蛋白-中华临床医师杂志.PDF

·8286 · 中华临床医师杂志( 电子版)2013 年9 月第7 卷第18 期 Chin J Clinicians(Electronic Edition),September 15,2013,Vol.7,No.18 ·基础论著· 甲基乙二醛对胰腺癌PANC-1 细胞增殖 及凋亡蛋白表达的影响 张涓娟 蒲宇 李勇 沈成义 张小明 【摘要】 目的 探讨甲基乙二醛（MGO ）对胰腺癌 PANC-1 细胞增殖影响及其作用机制。方法 培 养PANC-1 细胞，用四甲基偶氮唑蓝（MTT ）比色法检测MGO 对PANC-1 细胞增殖的影响；Hoechst33258 染色细胞后显微镜下观察MGO 处理后PANC-1 细胞形态变化，Western blot 检测凋亡家族蛋白Bcl-2 、Bax 和Caspase-3 的表达变化。结果 MTT 检测发现MGO 对人胰腺癌PANC-1 有明显的抑制作用，且在一定 程度上呈剂量时间依赖性；MGO 处理48 h 后的PANC-1 细胞在Hoechst33258 染色后荧光显微镜下呈现典 型的凋亡细胞核固缩表现；Western blot 检测表明MGO 能显著降低Bcl-2 蛋白表达量而提高Bax 和Caspase-3 蛋白表达量。结论 MGO 能够有效抑制人胰腺癌PANC-1 细胞增殖并诱导其凋亡，其作用机制可能是通过 作用凋亡信号通路调控凋亡家族蛋白Bcl-2 ，Bax 和Caspase-3 表达进而诱导PANC-1 细胞凋亡。 【关键词】 胰腺肿瘤； bcl-2 相关X 蛋白质； 甲基乙二醛； Caspase-3 ； Bcl-2 Effect of methylglyoxal on proliferation of human pancreatic carcinoma (PANC-1) cells and expression of apoptotic proteins ZHANG Juan-juan, PU Yu, LI Yong, SHEN Cheng -yi, ZHANG Xiao -ming. Sichuan Key Laboratory of Medical Image, North Sichuan Medical College, Nanchong 637000, China Corresponding author: ZHANG Xiao-ming, Email: cjr.zhxm@ 【Abstract 】 Objective To study the influence of methylglyoxal on human pancreatic carcinoma PANC-1 cells proliferation and possible mechanism. Methods Cell proliferation was evaluated using MTT method; Hoechst33258 staining assay was used to observe MGO-induced morphological changes; Changes for Bcl-2 and Bax expression level were assessed using Western blot. Results Comparing with control group, PANC-1 cells proliferation was suppressed by methylglyoxal. The growth inhibition induced by MGO was in a dose-dependent manner(P ＜0.01). Apoptosis of the PANC-1 cells was observed after exposure to MGO, which was verified by morphological changes and increased proportion of Hoechst positive cells. The expression level of Bcl-2