基于光镊拉曼光谱技术的葡萄球菌黄素生物合成的研究-理论物理专业论文.docxVIP

Study Study on biosynthesis of staphyloxanthin in staphylococcus aureus using laser eezers Raman SDeCtr0SCODV - I¨ Graduate student：Kang Jian Supervisors：Profs．Jun—xian Liu，Zhan—hua Tao Specialty：Theoretical Physics Direction：Biophysics Entrance year：20 1 3 Abstract Staphylococcus aureus is a kind of important clinical pathogenic bacteria,and the infection caused by MRSA，together with hepatitis B and AIDS have become three main infection chronic diseases in the world．The typical staphylococcus aureus is spherical，and in the shape of grape cluster under microscope，that is why it is called ”staphylococcus”．Meanwhile，because it Can generate a kind of golden carotenoid,it earned the name of staphylococcus aureus．111is golden carotenoid staphyloxanthin enhanced bacterial resistance ability of oxidative stress and the phagocytosis of host neutrophile granulocyte，which Can help bacteria escape from host inlmune system and establish close connection with bacterial virulence．Therefore，the blocking of the biosynthesis of staphylococcus flavin may become new effective means of treating MRSA infection as expected． The efficient and accurate detection of staphylococcus flavin in staphylococcus aureus is the precondition for understanding its mechanism of toxication as well as biosynthesis，and screening new antibiotics．However,the operation of traditional quantitative methods based on solvent extraction is complicated and toxic methyl alcohol is used．Laser Tweezers Raman Spectroscopy(LTRS)combined optical tweezers and Raman spectroscopy；it imprisons the living cells in solution by suing highly converged laser beam；then，it stimulates the molecular Raman scattering inside cells through increasing the light beam of imprisoned cells instantaneously． This technology fully combined诹m the advantages of confocal microscopy，optical III 万方数据 tweezers tweezers and Raman spectroscopy,and improved detection sensitivity as well as accuracy,which is the powerful tool for researching chemical