催乳素对外周血T细胞巨噬细胞移动抑制因子表达的影响-江苏大学.PDFVIP

18 1 ( ) Vo.l 18 No. 1 2008 1 Journal of Jiangsu Un iversity( M ed icine Ed ition) Jan. 2008 T 郭继强, 宋向凤, 牛志国 (, 53003) [ ] : ( PRL) T ( macrophage m igration inh ib ition factor, M IF): Ficol-lHypaque, T(PHA, 10 L g/m l)(PMA, 5L g/m l) T, ( 20, 300, 1 000 ng /m l) 2 h, RT-PCR T M IF ; EL ISA TM IF M IF ( pG l3-Basic-M IF) T, : M IF M IF M IF-lucr: T [ ] ; T; ; [ ] R392. 1 [ ] A [] 1671- 7783( 2008) 01- 0030- 0 Effects of prolactin regulating the expression ofmacrophagemigration inhibition factor derived from human peripheral bloodT cell G UO J i-qiang, SON G X iang-f eng, N IU Zh i-g uo (D epartment of Immunology, X inxiangM edicalCollege, Xinx iang H enan 53003, China) [Abstract] Objective: To study regu lating effects of prolactin on the expression and secretion ofM IF in hum an peripheralbloodT cells. Methods: Mononuclear cellsw ere obtained from human periphera blood by them ethod of Ficol-lH ypaque density grad ient centrifugation. T cellsw ere collected by nylon collumn. PHA ( 10 Lg /m l) and PMA ( 5 Lg/m l) w ere added into the med ium to active theT cells, D ifferent concentration PRL ( 20, 300, 1000 ng/m l) w ere app lied to T cells at the sam e tmi e. T cellsw ere collected after cu ltured for 2 h, the expression level ofM IF gene w as detcted by RT-PCR and the content ofM IF in T cell super- natantw as assayed by ELISA. M IF-luc report genew ere transfected into each group thatmetiond above, the relative luciferase activity ofM IF-luc report gene w as tested by kit