细菌性纤维素复合生物聚合物对其细胞增生与物理性质之影响-张文硕.DOC

國立宜蘭大學食品科學研究所 碩士班專題討論 題目：細菌性纖維素複合生物聚合物對細胞增生與物理性質之影響 Effect of biopolymers composition on physical properties and cell proliferation of bacterial cellulose 演講者：張文碩(Chang, Wen-Shuo) 指導教授：陳輝煌 老師 日期：Nov. 2, 2011 中文摘要 褐藻膠、細菌細纖維素、明膠和幾丁聚醣等天然聚合物，在過去十年已被研究為新的生醫材料替代品。細菌性纖維素具有生物相容性、3D網狀結構、高機械強度和高保水能力。經均質與凍乾所製成的細菌性纖維素海綿組織，其具有高表面積(92.81 ± 2.02 m2/g)及足夠的孔隙度(90.42±0.24%)，可促進滑膜間質幹細胞生長。比較纖維母細胞(NIH/3T3)在幾種天然物薄膜上的增生速度，其中以明膠薄膜優於細菌性纖維與褐藻膠，因此明膠適合應用於皮膚組織再生。作為非貼附型的水膠敷料，純細菌性纖維與細菌性纖維素/褐藻膠複合物(BCA)在體外試驗中，除了對人類角質形成細胞與牙齦纖維母細胞皆有增生效果外，BCA組織在濕態下具有良好的抗撕裂性。另外，當細菌性纖維素吸附幾丁聚醣或明膠形成複合薄膜後，具有互連的多孔網狀結構和大的比表面積，且可使結晶度下降與熱穩定增加，由細胞貼附試驗顯示，細菌纖維素複合物比純纖維有較高的細胞貼附性，促進細胞增生。 關鍵字：細菌性纖維素、褐藻膠、生物相容性、明膠 Abstract For the past ten years, biopolymers such as alginate, bacterial cellulose (BC), gelatin and chitosan have been studied for the use of new alternative biomedicine materials. BC possesses biocompatibility, 3D network structure, high mechanical strength and high water holding capacity. BC sponges were obtained through homogenizing and freeze-drying process, possessed high surface area (92.81 ± 2.02 m2/g), sufficient porosity (90.42 ±0.24%) and could promote stem cells (MSCs) proliferation. Comparison of several biomaterials proliferation rate of NIH/3T3 cells on membranes, the gelatin was superior to BC and alginate membrane. Therefore, gelatin is suitable to apply as a skin tissue regeneration template. As a non-adherent hydrogel dressing, pure BC and the BC/Alginate (BCA) sponges proliferated human keratinocytes (HaCat) and gingival fibroblasts (GF) in vitro, besides the BCA sponge exhibited desirable tear resistance in the wet state. In addition, the composites membranes, BC/chitosan and BC/gelatin, own fine interconnected porous network and large specific surface area, and can decrease crystallinity as well as improve thermal stability of BC. Cell attachment study results indicate that BC composites posses higher cell adhesion than BC could promote cell proliferation. Keywords: bacterial cellulose, alg