间充质干细胞对肝肿瘤细胞力生物学行为的影响及相关机理研究-生物学专业论文.docxVIP

重庆大学 重庆大学硕士学位论文 英文摘要 PAGE PAGE VI ABSTRACT MSCs possess critical properties, exhibit self-renewal ability and multi-lineage differentiation potential which can differentiate to osteoblasts, chondrocytes, adipocytes and myocyte under various culture conditions. As MSCs can be recruitted to the environment of inflammation and the sites of tissue injury, these cells play a significant role in repair and regeneration of tissue damage through a combined effect of anti-inflammatory, paracrine, proliferation and differentiation. The use of MSCs for gene delivery and cell therapy has been regarded as an important tool. Recently, MSCs have become the most widely used stem cell type which in tissue repair and regeneration. The malignant tumor was caused by the disorder of cell cycle or the unfetter cell divide and growth, it threatens human health seriously, however, there is still a huge challenge against the prevention and treatment of this disease. Inflammatory microenvironment was one of the most important characteristics of tumor tissues. Considering the fact that the normal tissue accompanied by malignant change also could be treated as a special kind of tissue damage. So tumor tissue can recruit MSCs to involve in remodeling the microenvironment of tumor, which further significantly influenced the biological behavior of tumor cells. In the area of tumor studies, the interactive dialogue and influences between tumor cells and MSCs will be a hot topic in the future. However, the mechanism of this phenomenon is not completely clear. So our research mainly focuses on the changes of biological behavior of rat hepatoma cells (CBRH-7919) in a microenvironment of co-culturing with MSCs and discusses the possible signaling pathway. The main content of this research and results are as follows: Isolation and culture of cell Percoll density gradient centrifugation and adherent culture was used to separate MSCs. The cells were maintained in L-DMEM supplemented with 10% fetal bov