甲状腺激素水平及其受体TRβ基因突变在甲状腺癌发病及预后中的作用-病理学与病理生理学专业论文.docxVIP

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甲状腺激素水平及其受体TRβ基因突变在甲状腺癌发病及预后中的作用-病理学与病理生理学专业论文.docx

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II II Abstract Objectives:（1）To assess a possible correlation between thyroid hormone and thyroid cancer. （2）To characterize a possible correlation between the TRβ gene exon 7 mutation and thyroid cancer. Mothods（: 1）The blood serum of thyroid hormone was assayed by electrochemiluminescence in 36 thyroid cancer, 42 benign lesion and 30 normal subjects. （2）The tissue of thyroid hormone was assayed by immunohistochemical staining in 48 thyroid cancer, 45 benign lesion and 16 normal subjects. （3） Polymerase chain reaction - single strand conformation polymorphism analysis (PCR-SSCP) method and DNA sequencing technology, based on conformational change of TRβ gene exons 7 mutation detection in 82 cases of thyroid cancer. Enumeration data mainly use χ2 analysis and Logistic recurrence analysis, measurement data mainly use one-factor analysis of variance and multiple linear regression.Size of test is α=0.05. Results: （1） ① The level of FT3 and FT4 in thyroid cancer are both lower than in that benign lesion and control group（P〈0.001）. ②The level of T3 and T4 are nonsense between three groups（P〉0.05）. ③ The FT3 level is inverse proportion with tumor pathology staging and lymphoid node metabasis.（2）①FT3 expression is sense in thyroid cancer, benign lesion and normal subjects（P0.05）. ② the positive rate of FT3 in thyroid cancer is 70.8%,which is lower than in that benign lesion 91.1% and control group100%,. difference is sense（P0.0125）.（3）82 cases of thyroid cancer in three cases of detected mutations: exon 7 at codon 1028 by a C→A missense mutation of the mutant amino acids so that mutation of aminothiopropionic acid to aminopropionic acid; exon 7 at codon 980 of the C→G missense mutation, mutant amino acids so that phenylalanine mutation to aminoacetic acid;exon7 at codon 1038 of the A→T missense mutation, mutant amino acids so that aminopropionic acid mutation to threonine. Conclusions: （1）The difference of the T3 level is sense ,means that low T3 level may play a certain