从随机突变到精确编辑果蝇基因组编辑技术的发展及演化-科学.PDF

Hereditas (Beijing) 2016 1 , 38(1): 1727 1 1 2 1,2 1 1,3 1 1., 511436 2. 100101 3. 361005 (Drosophila melanogaster) 20 (Ethyl methanesulfonate, EMS) CRISPR/Cas9(Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein) ZFN(Zinc-finger nucleases)TALEN(Transcription acti- vator-like effector nucleases) CRISPR/Cas9 ZFN TALENCRISPR/Cas9 From random mutagenesis to precise genome editing: the development and evolution of genome editing techniques in Drosophila 1 1 2 1,2 1 1,3 Fang Su , Zongliang Huang , Yawen Guo , Renjie Jiao , Zi Li , Jianming Chen , Jiyong Liu1 1. Sino-French Hoffmann Institute, Guangzhou Medical University, Guangzhou 511436, China; 2. State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; 3. State Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, State Oceanic Administra- tion, Xiamen 361005, China Abstract:Drosophila melanogaster , an important model organism for studying life science, has contributed more to theresearch of genetics, developmental biology and biomedicine with the development of genome editing techniques. Drosophila genome-editing techniques have evolved from random mutagenesis toprecise genome editing and