尖吻蝮蛇毒金属蛋白酶AaHIV及其结构域免疫效果评价-急诊医学专业论文.docxVIP

第三军医大学硕士学位论文 第三军医大学硕士学位论文 万方数据 万方数据 英文缩略表 缩写 ADAM 英文全称 a disintegrin and metalloproteinase 中文全称 解聚蛋白样金属蛋白酶 ANOVA analysis of variance 方差分析 Bp base pair 碱基对 BSA bovine serum albumin 牛血清白蛋白 BCA bicinchonininc acid 二喹啉甲酸法 CD catalytic domain 催化结构域 CFA complete Freund’s adjuvant 完全弗氏佐剂 KD Kilodalton 千道尔顿 ddH2O double distilled water 双蒸水 DNA deoxyribonucleic acid 脱氧核糖核酸 E.coli escherichia coli 大肠杆菌 ED50 EDTA median effective dose ethylenediaminetetraacetic acid 半数有效量 乙二胺四乙酸 ELISA enzyme-linkedimmunosorbent assay 酶联免疫吸附测定 EU ELISA unit ELISA 单位 EP eppendorf 微量离心管 HVR hypervariable region 高变区 IFA incomplete Freund’s adjuvant 不完全弗氏佐剂 IPTG isopropyl-1-thio-β-D-galactoside 异丙基硫代-β-D 半乳糖苷 Kana Kanamycin 卡那霉素 LB Luria-Bertanin broth LB 培养基 LD50 median lethal dose 半数致死量 MHD minimum hemorrhagic dose 最小出血剂量 NCD non-catalytic domain 非催化结构域 OD optical density 吸光度 PAGE polyacrylamide gel electrophoresis 聚丙烯酰胺凝胶电泳 PBS phosphate buffered saline 磷酸盐缓冲液 1 PMSF Phenylmethanesulfonyl fluoride 苯甲基黄酰氟 SDS sodium dodecyl sulfate 十二烷基硫酸钠 SVMP snake venom metalloproteinase 蛇毒金属蛋白酶 TAE Tris/acetate/(buffer) Tris/乙酸电泳缓冲液 TE Tris/EDTA buffer Tris-EDTA 缓冲液 TEMED N,N,N’,N’-tetramethylethylene Diamine 四甲基乙二胺 U unit 单位 Μl microliter 微升 X-Gal 5-bromo-4-chloro-3-indolyl-β-D-galactoside 5-溴-4-氯-3-吲哚-β-D 半乳糖苷 2 Evaluation of protective effects of antivenom induced by AaHⅣ and its domains from Agkistrodon acutus Abstract Background Administration of antivenom is the only effective way to neutralize the toxicity of snake venoms, and is strongly recommended by World Health Orgernization. To date, all of the antivenoms around the world are manufactured by hyperimmunizing horse or sheep. However, snake venom is a complex mixture of proteins and polypeptides, some toxic components have strong antigenicity, but no or very weak immunogenicity. And some have strong immunogenicity, but no or very weak antigenicity. Despite the purity of these antivenoms are much higher than them used to be,severe adverse reactions,