甲状腺乳头状癌BRAFT1799A基因突变与血小板衍生生长因子关系的研究-内分泌与代谢专业论文.docxVIP

摘要目的：BRAF 摘要 目的：BRAF T179鲋突变可能影响甲状腺癌生长方式，进而影响患者预后。血 小板衍生生长因子(PDGF)、血管内皮生长因子(VEGF)与肿瘤的转移密切相关。 本研究主要探讨乳头状甲状腺癌(PTC)患者BRAF T179鲥突变与PDGF、VEGF 表达及肿瘤生长方式的关系。 方法：实验对108名PTC患者的基因进行测序，并随机选取BRAF T1799．4阳 性和阴性病例各30名。BRAF T179鲥突变采用聚合酶链反应．聚丙烯酰氨凝胶电泳 法分析，产物经测序证实。PDGF、VEGF表达采用免疫组织化学方法检测。 结果：108名PTC患者中BRAF T1刀鲥阳性者有54名，阳性率为50．0％。BRAF T1799A阳性者肿瘤腺体外浸润和肿瘤III级以上分级发生率高于阴性患者(pO．05)。 BRAF79鲋患者PDGF．B表达水平明显高于阴性患者(p0．01)，而两者PDGF—A 和VEGF表达水平无明显差异(pO．05)。在调整性别、年龄后Logistic回归分析显 示BRAFTl79鲋与肿瘤腺体外浸润和肿瘤III级以上分级及PDGF．B表达呈明显正相 关。 结论：BRAF T1 79鲥影响肿瘤的生长方式，使PTC肿瘤腺体外浸润生长发生率 高，进而影响PTC患者临床预后。 关键词：BRAF T1977A 甲状腺乳头状癌 血小板衍生生长因子 血管内皮生长因子 AbstractObjective：BRAF Abstract Objective：BRAF T1 799A mutation may affect the growth style of thyroid and the prognosis of patient．Platelet—derived growth factor(PDGF)and vascular endothelial growth factor (VEGF)are closely correlated with tumor metastasis．This study mainly investigate the relation of BRAF Tl 799A mutation of papillary thyroid carcinoma(PTC、with expression of PDGF,VEGF and tumor growth style． Method：The study analyzed the gene of 1 1 6 PTC patients，and random selected 30 BRAF T1 799A positive and negative patients respectively．BRAF T1 799 mutation was analyzed with polymerase chain reaction-SSCP method，and the production was confirmed by gene sequencing．The expression of PDGF and VEGF was analyzed by immunohistochemistry． Results：There were 54 BRAF T1 799A positive patients in the 1 08 patients．The rate of positive was 50．0％．The BRAF T1 799A(+)PTC had higher rate of extra—thyroidal invasion and Ill／IV tumor stage when compared to BRAF T1 799．4(一)PTC(矿O．05)．Although BRAF T1 799．4(+)PTC tissues had higher expression levels of PDGF—B than BRAF T1 799A(-)PTC tissues，expression levels of PDGF-A and VEGF in BRAF V600E(+)PTC were not significantly different from those of BRAF V600E(-)PTC p0．05)．After adjustments for age，sex，Multivariate logistic regression analyses show BRAF T1 799A was positively correlated to extra—thyroidal invasion，Ill／IV tumor stage and expr