分光光度法测定肉苁蓉多糖含量-食品安全质量检测学报.PDF

第8 卷 第7 期 食品安全质量检测学报 Vol. 8 No. 7 2017 年7 月 Journal of Food Safety and Quality Jul. , 2017 分光光度法测定肉苁蓉多糖含量 李 莉, 曹 进* ( 中国食品药品检定研究院, 北京 100050) 摘 要: 目的 建立一种分光光度法测定肉苁蓉多糖含量的方法。方法 肉苁蓉多糖经水提醇沉提取后, 在 浓硫酸作用下水解成单糖, 并迅速脱水生成糠醛衍生物, 采用蒽酮-硫酸分光光度法在627 nm 处测定其含量。 结果 肉苁蓉多糖质量浓度在20~100 μg/mL 范围内线性关系良好, 方法平均回收率在93.0%~99.8%之间, 相 对标准偏差(relative standard deviation, RSD)为 1.7%~4.5%。对肉苁蓉、管花肉苁蓉和盐生肉苁蓉3 种肉苁蓉 中多糖含量进行了测定, 结果表明3 者的多糖含量存在差异。结论 本方法简便、准确, 可用于肉苁蓉多糖含 量的测定。 关键词: 肉苁蓉; 多糖; 分光光度法 Determination of polysaccharides in Cistanehe herba by spectrophotometry * LI Li, CAO Jin (National Institutes for Food and Drug Control , Beijing 100050, China) ABSTRACT: Objective To establish a method for the determination of polysaccharides in Cistanehe herba by spectrophotometry. Methods The polysaccharides were extracted with water and precipitated by alcohol. Then they were hydrolyzed into monosaccharides by sulfuric acid which was rapidly dehydrated into furaldehyde. The content of polysaccharides was determined by anthrone-sulfurine acid spectrophotometric method, and was measured at 627 nm. Results The standard curve of polysaccharide showed a good linear relationship at the range of 20~100 μg/mL. The average recoveries were 93.0%~99.8%, and the relative standard deviations were 1.7%~4.5%. There were differences in polysaccharide contents among 3 d