羽衣甘蓝MADS–box基因BoaAGL6的克隆及表达分析.PDF

() 2014 10 40 5 Journal of Hunan Agricultural University (Natural Sciences), Oct. 2014, 40(5):487–493 DOI:10.13331/ki.jhau.2014.05.007 /qks 羽衣甘蓝 MADS–box 基因 BoaAGL6 的克隆及表达分析 1,2 1,2 1,2 1,2* (1. 1500402. 150040) 摘 要(Brassica oleracea var. acephala)2 RACE AGL6 BoaAGL6 RT–PCR PCR BoaAGL6 3 BoaAGL6 999 bp(GenBank KC984301)759 bp252 BoaAGL6 AGL6–like RT–PCR RT–PCR BoaAGL6 2 关 键 词MADS–box BoaAGL6 中图分类号S635.9 文献标志码：A 文章编号：1007− 1032(2014)05−0487−07 Cloning and expression analysis of MADS–box gene BoaAGL6 associated with floral development in kale (Brassica oleracea var. acephala) HAN Yue1,2REN Hong-jie1,2ZHAI Xiao-yu1,2XU Qi-jiang1,2* (1.The State Key Laboratory of Tree Genetics and Breeding, Harbin 150040, China; 2.College of Life Sciences, Northeast Forestry University, Harbin 150040, China) Abstract: Wild type kales (Brassica oleracea var.acephala) and its two mutants which have different petals in number were used as material. One AGL6 -homologue from conventional kale varieties denoted as BoaAGL6 was cloned using RACE (rapid amplification of cDNA ends), then expression pattern of BoaAGL6 in each floral organ was examined. The results showed that BoaAGL6 contains a 759 bp open reading frame that encodes a deduced protein with 252 amino acid residues. Phylogenetic analysis indicated that BoaAGL6 belongs to the AGL6 -like subfamily. The result from multiple methods (semi-quantitative RT–PCR and quantitative real-time PCR) revealed that the pattern of the expression of BoAGL6 is broader than those of their counterparts in eudicots, with