降解芦苇木质素菌种的选育及在制浆中的应用研究-遗传学专业论文.docxVIP

内蒙古 内蒙古科技大学硕士学位论文 III III optimal conditions for the bleaching of pulp were shown: pulp concentration 4%, hydrogen peroxide 5%, EDTA 2%,hydrogen peroxide1%, temperature 60℃ and reaction time 2 h. The whiteness of pulp reached 48.9% and was increased by 8.3% ISO, compared with the former whiteness 40.6%. Through the pretreatment of paper pulp bleaching by laccase, xylanase and laccase/xylanase, the pulp brightness could reach 52.1%, and with the mixing ratio optimized and the ratio of laccase: xylanase to 2:1, the whiteness was 59.6%. The whiteness was improved by 7.5% ISO, compared with the paper pulp without enzyme pretreatment. Through the detection of bleaching effluent, CODCr value of waste water was reduced and at the same time pH value showed the alkalescence by the utilization of enzyme. That is to say, the removal rate of CODCr by mixed enzymes was reduced by 20.01%, compared with that of bleaching effluent which had no pretreatment of enzymes, and the pH value was lowered from 10.3±0.2 to 8±0.2. It comes to the conclusion that the mixed strains have obvious effects on lignin degradation and their mixed enzymes show the distinct effects for pulp bleaching, which will have important significance in biopulping. Key Words：Phragmites australis；Lignin；Biobleaching；Laccase；Xylanase PAGE PAGE 1 目 录 摘 要 I Abstract II 1 文献综述 1 1.1 造纸原料 1 1.1.1 芦苇 1 1.1.2 芦苇在造纸中的研究进展 1 1.2 木质素降解菌和降解酶系 2 1.2.1 木质素 2 1.2.2 降解木质素的微生物 3 1.2.3 降解木质素的酶系及其降解机理 4 1.2.4 混合菌种降解木质素 5 1.3 纸浆漂白 6 1.3.1 螯合处理 6 1.3.2 过氧化氢漂白 7 1.3.3 生物漂白 8 1.4 制浆工艺 11 1.4.1 化学机械制浆 11 1.4.2 生物机械制浆 11 1.4.3 生物化学制浆 12 1.5 本课题的研究目的和基本内容 12 1.5.1 研究意义 12 1.5.2 研究的基本内容 13 2 降解木质素菌种筛选 14 2.1 实验仪器与试剂 14 2.1.1 实验仪器 14 2.1.2 实验试剂 15 2.2 培养基配制 16 2.3 实验方法 16 2.4 结果与分析 16 2.5 小结 17 3 普通青霉菌产漆酶条件优化及酶学性质研究 18 3.1 实验仪器与材料 18 3.1.1 实验仪器 18 3.1.2 实验材料 18 3.2 实验方法 18 3.2.1 漆酶酶活测定 18 3.2.2 产酶条件的研究 19 3.2.3 酶学性质的研究 20 3.2.4 正交实验优化 20 3.2.5 蛋白含量的测定 21 3.3 结果与分析 22 3.3.1 不同碳源对普通青霉菌产漆酶的影响 22 3