玉米不同组织器官谷氨酰胺合成酶同工酶表达差异及聚合方式-作物学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2017, 43(9): 14101414 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2017.01410 玉米不同组织器官谷氨酰胺合成酶同工酶表达差异及聚合方式 王小纯1,2,3 张浩然3 韦一昊1 贾喜婷3 谷明鑫3 马新明1,* 1 , 450002; 2 , 450002; 3 , 450002 : (GS), GS , GS Western blot , GS , 40 kD GS1 , 39 kD GS1 , 44 kD GS2 BNE , , GS ; 2-D Western blot GS , 3 GS , GS2 460 kD, ; GS1 2 , 410 kD , 240 kD , GS : ; (GS); ; BNE; Differential Expression and Assembly Mode of Glutamine Synthetase Isoen- zymes in Different Tissues and Organs of Maize 1,2,3 3 1 3 3 1,* WANG Xiao-Chun , ZHANG Hao-Ran , WEI Yi-Hao , JIA Xi-Ting , GU Ming-Xin , and MA Xin-Ming 1 Collaborative Innovation Center of Henan Grain Crops / Henan Agricultural University, Zhengzhou 450002, China; 2 State Key Laboratory of Wheat and Maize Crop Science in China / Henan Agricultural University, Zhengzhou 450002, China; 3 Department of Biochemistry, College of Life Science / Henan Agricultural University, Zhengzhou 450002, China Abstract: Glutamine synthetase (GS) is a key enzyme in nitrogen assimilation and recycling in cereals. In this study, the expres- sion characteristics of GS isoenzymes in different tissues and organs of maize in grai