流式细胞术在细菌快速检测中的应用-微生物学报.PDF

微生物学通报 Jan. 20, 2014, 41(1): 161−168 Microbiology China © 2014 by Institute of Microbiology, CAS tongbao@ DOI: 10.13344/j.microbiol.china.130041 专论与综述 流式细胞术在细菌快速检测中的应用 刘新星 霍转转 云慧 杨英杰* ( 410083) 摘 要流式细胞仪(Flow cytometer)是集应用流体学、光学、电子学、生物学、免疫学等多门 学科和技术于一体的新型高科技仪器。它的核心技术是流式细胞术(Flow cytometry ，FCM) ，该 技术是利用流式细胞仪，使单个细胞或其他微小生物粒子处于快速直线流动状态，且逐个通过 光束，从而对单个细胞或微粒进行多参数(数量、大小、核酸含量、细胞活性、特定菌群或物 种等)定量分析和分选的检测技术，具有快速、灵敏、精确以及便于操作等突出优点。本文简 要介绍流式细胞仪的原理，并论述流式细胞技术在实验室研究、工业生产、临床诊断、环境评 估等领域的细菌快速检测应用。 关键词流式细胞术，工作原理，细菌，快速检测，应用 Application of flow cytometry to rapid detection of bacteria * LIU Xin-Xing HUO Zhuan-Zhuan YUN Hui YANG Ying-Jie (Key Laboratory of Biometallurgy of Ministry of Education , School of Mineral Processing and Bioengineering, Centre South University, Changsha, Hunan 410083, China) Abstract: Flow cytometer, an advanced instrument integrating fluidics, optics, electronics, biology, immunology, etc., measures cells in suspension that flow in single-file through laser beam. Flow cytometry (FCM), as the core of flow cytometer, is a multidisciplinary technique for analysing and sorting cells or other particles. FCM can provide a multitude of information at the single-cell level, including (but not limited to) total counts, size measurements, nucleic acid content, cell viability and activity, and detection of specific bacterial groups or species. The main advantage of FCM is that it is fast, sensitive, accurate and easy to perform. Here, we briefly