金纳米棒的制备及其在检测人体端粒DNA构型变化中的应用-化学 有机化学专业论文.docxVIP

AbstractGold Abstract Gold nanoparticle and gold nanorods(AuNRs)have attracted much attention in recent years due to their unique structural and optical properties．AuNRs have tunable anisotropic structure and near infrared(NIR)absorbtion，which is appliable in several fields，such as metal ions detection，immunoassay,DNA sequencing， cancer cell imaging，light-heat treatment and nano—meterial assembling．Human telomere consists of tandem repetitive sequence of(TTAGGG)n and plays an important role in anti-cancer drug selection．The applications of AuNRs in monitoring G．quadruplex DNA conformation change Was studied for potential anticarcinogen screening．This paper includes the flowing four pans： 1．Gold nanoparticles with different sizes and AuNRs with different aspect ratios were synthesized and their optical properties were studied．AuNRs with optical absorption matches the emission spectra of fluorescent dye were prepreapred，which allows the FRET process to take place．Gold nanoparticle and AuNRs dispersed in organic solvet were also prepared． 2．A facile and multi—response strategy for studying the transformations of human telomere DNA from single strand(SS)to double strand(ds)and G-quadruplex has been established by using positively charged gold nanorod(AuNR)as all optical label．The conformation change information of the telomere DNA Was transferred into multiple optical signals，including changes in fluorescence emission，near infrared(NIR)absorption，plasma resonance light scattering(PRLS)and dynamic light scattering(DLS)response．The formations of dsDNA and G。quadruplex DNA induced fluorescence quenching of dye on DNA,and were accompanied by the intensity decrease and blue shiR of the longitudinal absorption peak of AuNRs． Meanwhile，PRLS and DLS results revealed slightly increased AuNR aggregation due to increased charge density of dsDNA and G—quadruplex DNA as compared to ssDNA．Control experiment suggests that the AuNR-based assay is highly sequence specific；and the high s