镜鲤酸性磷酸酶和碱性磷酸酶的QTL分析-水产养殖专业论文.docxVIP

上海海 上海海洋大学硕士学位论文 II II LG14（HLJ3275-CA174）和（CA1276-CA2208）、LG25（HLJ2941-HLJ3946），95% 置信区间分别为 0-10cM、84-86cM、96-103cM 和 71-84Cm,可解释表型变异分别为 10.0%、8.3%、8.1%和 9.9%。 3. 肝脏组织碱性磷酸酶活性连锁的微卫星标记的 BLAST 比对结果 通过 BLAST 将与肝脏 ALP 活性相关的 QTL 两端的 SSR 标记与斑马鱼全基因 组序列数据库进行序列比对，取期望值≤1e-10 的基因注释，结果显示：第 8 连锁群 定位的 QTL 区间两端的微卫星标记 HLJ1897 和 HLJ1244 分别与斑马鱼 13 号染色 体的 NW001877335.3 和 NW001877356.3 同源；同样比对第 41 号连锁群定位的 QTL 区 间 两 端 微 卫 星 标 记 HLJ3948 和 HLJ495 分 别 与 斑 马 鱼 13 号染色体 NW 001877341.3 和 NW 003040144.2 同源。LG8 和 LG41 连锁群上发现的 QTL 区间两 端微卫星分子标记与斑马鱼全基因组比对结果显示，两个区间在比对斑马鱼染色 体上有一个共同区间，在这一共同区间内，发现蛋白磷酸酶（protein phosphatase, Mg2+/Mn2+ dependent, 1Aa）与 ALP 活性相关，并在 LG8 对应的斑马鱼 13 号染色 体区间找到无机磷酸酶基因（Inorganic pyrophosphatase）。 本研究构建鲤鱼中等密度遗传连锁图谱，实现对肠组织酸性磷酸酶活性及肠 肝两组织中碱性磷酸酶活性 QTL 的初步定位，为鲤鱼分子标记辅助育钟和比较基 因组作图提供理论依据。 关键词：镜鲤，微卫星，碱性磷酸酶，酸性磷酸酶，遗传连锁图谱，数量性状基 因座 PAGE PAGE IV Mapping QTLs of Activity of Acid Phosphatase and Alkaline Phosphatase for Common Carp ABSTRACT In this research, a whole genome scan was conducted on 190 progenies from a full-sib family using microsatellite markers and a half-sib mapping strategy was employed to identify QTL associated with activity of acid phosphatase in intestinal and alkaline phosphatase traits in both intestinal and liver. The main results and major conclusion are as followings： 1. Construction of genetic maps In the study,a genetic linkage map has been constructed for the common car using JoinMap 4.0 software. 315 markers（28%） showed a significant distortion.The results showed that the framework of genetic linkage maps consisted of 992 SSR markers in 51 linkage groups. The total linkage distance spanned by these markers was 5183.9cM with an average of 5.22cM for the whole framework map and the map coverage was 88.8%. The biggest group was 51 linkage with 9 markers and the span was 148.8cM;the smallest group was 1 linkage with 9 markers and the span was 42.2cM;the 1 linkage with the highest high resolution was constructed with 40 markers spanning 72.4cM and the average was 1.85.