毕业银杏叶提取物加蝇蛆喂鸡诱导重要器官热休克蛋白表达研究.docxVIP

银杏叶提取物加蝇蛆喂鸡诱导重要器官热休克蛋白表达研究 摘要：目的：本次试验主要目的是探讨一种饲喂方案对鸡抗应激能力的影响，并 提出对生产实际有一定指导意义的饲料配方。方法：将一批受试鸡随机设置成6 组，乩40只雏鸡+普通饲料，b. 40只雏鸡+普通饲料+1%蝇蛆，c. 40只雏鸡+普 通饲料+0. 1%银杏叶提取物，d. 40只雏鸡+普通饲料+0. 3%银杏叶提取物，e. 40 只雏鸡+普通饲料+0. 6%银杏叶提取物，f. 40只雏鸡+普通饲料+0. 1%银杏叶提取 物+1%蝇蛆六个不同生境对照组。饲养45天后，取样，测定热应激后鸡热休克蛋 白含量，测定热应激后谷丙转氨酶、谷草转氨酶、肌酸激酶、肌酸激酶同工酶、 乳酸脱氢酶等血清生化指标，测定不同组热应激前后鸡热休克蛋白含量的变化。 结果：a饲料中添加银杏叶提取物加蝇蛆热休克蛋白表达的最多，f组差异极显 著。b血清生化指标明显降低，谷草转氨酶、肌酸激酶、肌酸激酶同工酶、差异 显著。C热应激前后热休克蛋白含量差异显著。结论：银杏叶提取物加蝇蛆喂鸡 可以很有效的诱导重要器官热休克蛋白的研究，对生产实际有非常重要的指导意 义。 关键词：银杏叶提取物；蝇蛆；诱导；热休克蛋白；表达研究 Ginkgo biloba extract increases the vital organs flylarvae feed the chickens induced expression of heatshock proteins 2007 Veterinary Medicine chen lulu (Academic Advisor: zhang miao) Abstract: Between February and March in 2011, chicks were fed with different percentages of Ginkgo biloba extract in Wensheng ecological breeding base, Haianxian, Nantong, Jiangsu province. All chicks were randomly divided into 6 groups, based on different habitat controls: a. 40 chicks with normal feed, b. 40 chicks with normal feed + 1% fly maggots, c. 40 chicks with normal feed and 0」％ Ginkgo biloba extract, d. 40 chicks with normal feed and 0.3% Ginkgo biloba extract, e. 40 chicks with normal feed and 0.6% Ginkgo biloba extract, f. 40 chicks with normal feed and 0」％ Ginkgo biloba extact and 1% fly maggots.. Observations on the growth and death in each group were recorded. After 30 days，feed, samples in each group were taken and the growth, quality and some serum biochemical indicators, including alanine aminotransferase (ALT), aspertate aminotransferase (AST) and urea nitrogen (BUN) were measured and recorded, together with some immunity indicators, such as Total protein, Albumin, Immunoglobulin etc. Analyses were made on the effects of six different kinds of feed to the growth, quality and some immune serum indicators. Key words: Ginkgo biloba extract; Myiasis; Induced; Heat shock protein ;Expression 0引言 银杏叶是一种具有很高药用价值的植物。银杏（学名:Ginkgo biloba）,别 名白果、公孙树，属裸子植物，和它同门的所有