同时蒸馏萃取-东西分析.PDF

现代科学仪器 第5期2010年10月 72 Modern Scientific Instruments No.10 Oct. 2010 同时蒸馏萃取-气相色谱/质谱法测定矢竹叶中的挥发性组分 1 1 2 刘聪 　汪雨 　刘学锋 1 2 （北京市理化分析测试中心/国产科学仪器应用示范中心 北京 100089； 北京动物园 北京 100044） 摘　要　采用同时蒸馏萃取法（Simultaneous Distillation and Extraction, SDE）提取人工圈养大熊猫取食矢竹叶中的挥发性组分， 然后经气相色谱/质谱联用技术（Gas Chromatography/Mass Spectrometry, GC/MS）对其进行分离、鉴定。实验共分离出了42种组分， 利用Nist数据库鉴定了其中31种，占矢竹叶挥发性组分总峰面积（总离子流图）的93.69%。其中主要成分为棕榈酸(44.00%)、 亚油酸 (24.40%)、（E）-2- 己烯醛 (4.10%)、苯并呋喃 (2.48%)、十一烷酸 (1.65%)、十三烷酸 (1.27%)、叶绿醇 (0.97%)、异 植醇(0.96%)、（E）-2-己烯醇(0.85%)、a-紫罗兰酮(0.70%)、苯甲醇(0.60%)、苯乙醛(0.50%)。 关键词　矢竹叶;挥发性组分;同时蒸馏萃取;气相色谱-质谱法 中图分类号　O657.7 J 1 1 2 Liu Cong , Wang Yu , Liu Xuefeng 1 ( Beijing Center for Physical Chemical Analysis, Application Demonstration Center for Homegrown Scientifi c Instruments, Beijing 100089, China; 2Beijing Zoo, Beijing 100044, China) Abstract The volatile components were extracted from leaves of Pseudosasa japonica by simultaneous distillation and extraction, and analyzed by gas chromatography-mass spectrometry (GC-MS). From total 42 components, 31 components were identifi ed, which accounted for 93.69% of the total peak area of the volatile components. Among them, the major components were palmitic acid (44.00%), linoleic acid (24.40%), (E)-2- Hexenal (4.10%), benzofuran (2.48%), undecanoic acid (1.65%), tridecanoic acid (1.27%), phytol (0.97%), isophytol (0.96%),(E)-2- Hexenol (0.85%), a- ionone (0.70%), Benzyl alcohol (0.60%), phenylacetaldehyde (0.50%). Key word