斑点叉尾鱼回鱼皮胶原的研究.pdf

DOI :10.13327/j.j jlau.2006.03.026 　28 3 　　　　　　　 Vol.28 No.3　 　2006 6 Journal 　of 　Jilin　Agricultural　University June 2006 　 刘海英, 李　 丁, 过世东 (江南大学食品学院教育部食品科学与安全重点实验室, 无锡 214036) 　:从斑点叉尾鱼回鱼皮中提取酸溶和酶溶胶原并对其进行了 分定性研究。 实验中获得的胶原中甘氨 酸最为丰富, 质量分数超过23%, 酸溶胶原的变性温度为32.5 ℃, 比猪皮胶原的变性温度低5 ℃。 SDS 实验结果表明酸溶胶原至少有2 条不同的α链。 酸溶胶原和酶溶胶原分别占原料干基的25.8% 和38.4%, 可以作为猪皮、牛皮胶原的替代品。 :斑点叉尾鱼回;胶原;氨基酸;变性温度 :S965.1　　　:A　　　:1000-5684(2006)03-0342-04 Studies on the Collagen of Channel catf ish Skin LIU Hai-ying, LI Ding, GUO Shi-dong (School of Food Science and Technology, Southern Yangtze University , Key Laboratory of Food Science and Safety, Ministry of Education, Wuxi 214036, China ) Abstract:Acid-soluble and pepsin-soluble collagens (ASC and PSC)were e tracted from the skin of Channel caft ish and partially characterized.The collagen obtained in the e periment contained more than 23%glycine and it is the most abundant amino acid.The denaturation temperature of acid-soluble colla- gen from the Channel caft ish was 32.5 ℃, about 5 ℃lower than that of the porcine skin collagen.SDS- PAGE showed that the collagens were composed of two distinct alpha chains.The ASC and PSC, on a dry weight basis, were 25.8%and 38.4%, respectively.These results suggest that Channel caft ish skin has potential as a supplement of the source of vertebrates collagen. Key words:Channel caft ish;collagen;amino acid;denaturation temperature 　　, 。, 、、、、、, , 。 , 、 , 。 、、、 。 、