外源性瘦素通过丝裂素活化蛋白激酶抑制猪骨骼肌成肌细胞分化.PDF

生 物 工 程 学 报 Chin J Biotech 2008, January 25; 24(1): 63-67 Chinese Journal of Biotechnology ISSN 1000-3061 cjb@ ©2008 Institute of Microbiology, CAS CSM, All rights reserved. 研究报告 于太永, 庞卫军, 吴江维, 卢荣华, 杨公社 , , 712100 : 研究瘦素(leptin)对猪骨骼肌成肌细胞分化的影响, 并探讨其可能的作用机制。原代培养猪骨骼肌成肌细胞, 通 过倒置显微镜定性观察细胞分化的形态学变化; 肌酸激酶(CK)活性测定法定量分析成肌细胞分化程度; 细胞免疫化学 方法分析myogenin 的表达情况; 免疫印迹技术(Western Blot)检测丝裂素活化蛋白激酶(mitogen activated protein kinase, MAPK) 的表达变化。结果显示, 在猪骨骼肌成肌细胞分化过程中, 外源性leptin 减少细胞核融合和肌管形成, 呈浓度和 时间依赖性地显著降低CK 活性( P 0.05), 并显著抑制myogenin 和MAPK 的蛋白表达( P 0.05) 。以上结果说明, leptin 抑制猪骨骼肌成肌细胞分化, 并且这种作用可能是通过激活MAPK 信号转导通路实现的。研究结果显示, leptin 在骨骼 肌成肌细胞分化过程中可能具有重要作用。 : 瘦素, 丝裂素活化蛋白激酶, 成肌细胞, 分化, 猪 Exogenous Leptin Inhibits Differentiation via MAPK in Swine Skeletal Myoblasts Taiyong Yu, Weijun Pang, Jiangwei Wu, Ronghua Lu, and Gongshe Yang Laboratory of Animal Fat Deposition and Muscle Development , Northwest AF University , Yangling 712100, China Abstract: We determined whether body-weight regulator, leptin, could influence differentiation of swine skeletal myoblasts. Myoblast occured in non-leptin and leptin formed in different concentrations (10~1000 ng/mL) for various duration (12~60 h). The morphologic assay demonstrated that leptin significantly decreased the formation of myotubes and myogenic index. In addition, bio- chemical analysis showed that leptin inhibited Creatine Kinase (CK) activity, expressions of myogenin and mitogen activated protein kinase (MAPK). As