用毛细管气相色谱法测定甲壳素的纯度-烟草科技.PDF

/ Tobacco Science Technology 2002 8 ( 181) 1,2 1 , (1.江南大学, 江苏省无锡市　214036; 2.中国科学技术大学烟草研究中心,合肥市美菱大道121 号　230052) 【】甲壳素;气相色 法;氨基葡萄糖盐酸盐 【】主要探讨了甲壳素纯度的测定方法。先用盐酸将甲壳素水解,然后将得到的水解产物——— 氨基葡萄糖盐酸盐(GAH)进行衍生化, 再用毛细管气相色 法(cGC 法)测定甲壳素的衍生物,并和比色 法进行了比较。结果表明:cGC 法是一种灵敏的测定甲壳素纯度的方法, 而比色法所得结果比期望值 低。 【】TS426　【】B　【】1002-0861(2002)08-0028-05 Determination Of Purity Of Chitin by Capillary Gas Chromatography ZHU XIAO-AN (1,2)and XIA WEN-SHUI(1) 1.School of Food Technology, Wuxi University of ight Industry, Wuxi, Jiangsu 　214036, China 2.Tobacco Research Center of University of Science Technology of China, Hefei 　230052, China Keywords:Chitin;Gas Chromatography;Derivatization;Glucosamine Abstract:A method for determining the purity of chitinwas mainly studied.First, a given amount of chitinwas hy- drolyzed in 8 mol/ hydrochloride solution at 110 ℃for 4 hours, and then the resulting glucosamine hydrochloride was reacted with N,O-bis(trimethylsilyl)trifluoroacetamide and 1%trimethylchlorosilane in the hydroxylamine so- lution of pyridine at 70 ℃for 20 minutes, and its derivative was analyzed by gas chromatography with a capillary column.The gas chromatography method was also compared with a colorimetric method for determining chitin.The result indicated that the gas chromatography was more sensitive and accurate than the colorimetry. 　　(chitin), , 、、、 β-(1,4)-2--2--D- ,、、、、 ,, [1-5] , , 。, 20 。 , 10 ,、 、、、 10%～30 %。 [1,