饥饿对幼年斑马鱼下丘脑摄食相关性神经肽表达的影响-遗传.PDFVIP

Hereditas (Beijing) 2016 年 9 月, 38(9): 821 ―830 研究报告 饥饿对幼年斑马鱼下丘脑摄食相关性神经肽表达的影响 刘姗姗，张翠珍，彭刚 复旦大学脑科学研究院，上海 200032 摘要 : 脊椎动物下丘脑中的神经肽 Y(Neuropeptide Y, NPY) 、GALANIN 和 GMAP 蛋白前体(GALANIN and GMAP prepropeptide, GAL) 、Agouti 相关蛋白(Agouti related neuropeptide, AGRP) 和阿片促黑色素原 (Proopiomelanocortin, POMC) 与摄食密切相关，但在斑马鱼中对这些神经肽与摄食之间关系的研究较少。本文 通过原位杂交技术和实时定量PCR 方法，观察饥饿 1 d 、饥饿2 d 和饥饿2 d 喂食2 d 后斑马鱼下丘脑中npy 、 galanin 、agrp 和pomca 的表达情况。结果显示，饥饿处理之后，agrp 和galanin 在斑马鱼下丘脑中的表达量显 著上升(P0.05) 。与对照组相比，饥饿2 d 后斑马鱼下丘脑中pomca 表达量显著下降(P0.05) 。饥饿2 d 喂食2 d 后斑马鱼下丘脑中pomca 、agrp 和galanin 的表达量与对照组相比没有显著性差异。所有实验中npy 在斑马鱼 下丘脑中的表达没有显著性差异。这表明饥饿处理促使斑马鱼下丘脑中agrp 和galanin 表达上调，pomca 表达 下调；及时摄食可以恢复agrp 、galanin 和pomca 在下丘脑中的表达水平。 关键词 : 斑马鱼；npy ；galanin ；agrp ；pomca Effects of starvation on the expression of feeding related neuro- peptides in the larval zebrafish hypothalamus Shanshan Liu, Cuizhen Zhang, Gang Peng Institute of Brain Science, Fudan University, Shanghai 200032, China Abstract : Vertebrate feeding behavior is regulated by neuropeptide Y (NPY), GALANIN and GMAP prepropeptide (GAL), agouti related neuropeptide (AGRP) and proopiomelanocortin (POMC) in the hypothalamus. However, there are few studies on the relationship between these neuropeptides and feeding in zebrafish larvae. In the present study, real-time quantitative PCR and in situ hybridization were applied to examine the expression levels of npy, galanin , agrp and pomca in the hypothalamus of zebrafish larvae after starvation and re-feeding. The results showed the expression of agrp and galanin increased significantly after starvation compared to the control group, whilst the expression of pomca decreased significantly compared to control. If the animals were re-fed for two days after starvation, the expression of pomca, agrp and galanin show