SSR荧光标记秋子梨种质分子身份证构建及亲缘关系分析.DOCVIP

SSR荧光标记秋子梨种质分子身份证构建及亲缘关系分析 基金项目：国家现代梨产业技术体系建设专项(CARS-28-01A) ; 中国农业科学院科技创新工程(CAAS-ASTIP-2017-RIP)；国家自然科学基金项目 作者简介：张小双，Tel:E-mail: xiaoshuangzhang@163.com。通讯作者：曹玉芬，Tel: 0429-3598125；E-mail: yfcaas@263.net. 张小双，曹玉芬*，齐丹，张莹，田路明，董星光，霍宏亮，徐家玉，刘超 （中国农业科学院果树研究所，农业部作物基因资源与种质创制辽宁科学观测实验站，辽宁兴城 125100） 摘 要：以“国家果树种质兴城梨、苹果圃”保存的53份秋子梨种质为试材，利用位于梨基因组17条染色体上多态性好的17对SSR荧光引物构建秋子梨种质分子身份证，建立每份种质的条形码标识，并探讨种质之间的亲缘关系。17对SSR引物在53份秋子梨材料中共检测到267个等位基因，平均每对引物检测到15.7个；NAUpy45b检测到的等位基因数最多为23个，NAUpy54b检测到的等位基因数最少为10个；所有引物的香农信息指数平均为2.311，其中NAUpy45b的香农信息指数最高为2.673；NAUpy86c的香农信息指数最低为1.493。聚类分析53份秋子梨可以划分成2个组，与地理相关；长白山及小兴安岭的秋子梨野生资源单独聚类，与栽培品种亲缘关系较远；“热梨”和“白八里香”可能为同物异名品种。SSR标记可以区分梨的芽变品种，可用于梨种质资源鉴别和保护。 关键词：梨；种质资源；SSR荧光标记；分子身份证；亲缘关系 Molecular Identification Establishment of Pyrus ussuriensis Maxim Accessions Using the Fluorescent-labeled SSR Markers and Genetic Relationship Analysis ZHANG Xiao-shuang, CAO Yu-fen*, QI Dan, ZHANG Ying, TIAN Lu-ming, DONG Xing-guang, HUO Hong-liang, XU Jiayu and LIU Chao (Research Institute of Pomology，Chinese Academy of Agricultural Sciences；Liaoning Research Station of Crop Gene Resource Germpiasm Enhancement, Ministry of Agriculture, P.R. China; Xingcheng，Liaoning 125100，China) Abstract Fifty three accessions of P. ussuriensis preserved in the National Repository of Pear and Apple Germplasm Resources in Xingcheng, China were used to establish the molecular ID and reveal their genetic relationship. Seventeen highly polymorphic fluorescent-labeled SSR makers which are located in 17 chromosomes of pear genome were selected to establish the molecular ID and bar code ID. A total of 267 alleles were detected with an average of 15.7 per locus, with the NAUpy45b had the highest number (23) and the NAUpy54b had the lowest (10). The average Shannon information index was 2.311, with the NAUpy45b showed the highest number (2.673), whereas the NAUpy86c showed the lowest (1.493). 53 accessions of P. ussuriensis can be divided into 2 classes by cluster analysis.