粉刺侧孢霉产木质素过氧化物酶酶活性与pH值的关系.PDF

1997 10 8 5 CH INE SE J OU RNA L OF APPL IE D E COL OGY , Oct . 1997, 8( 5)%527 ~ 5 30 pH * 丁佐龙 高 慧 ( , 230036) (Phanerochaete chrysosporium) C , pH . 37~ 39!C , , pH . , pH 6 . 0 0. 126 U ∀ml- 1, C , pH . . pH Relationship between lignin peroxidase activity of P ha nerochaete chry sosp orium and pH value. Ding Zuolong and Gao Hu i(A nhui Agricultural University, Hef ei 230036) . Chin. J. App l. Ecol. , 1997, 8( 5) 527~ 5 30 . Phanerochatet chrysosp orium w as liquidcultur ed w ith avicel as car bon source and w ith veratric alcohol as enzy m eindu cing agent . Deter m ination s of it s lignin p erox idase activity at diff erent initial pH values show ed that the hig hest enzym e activity w as obtained at pH 6 . 0 , indicating that the o ptimum pH value w as relatively higher w hen avicel w as u sed to subst itute g lucose as a carbo n source. T he enzym e activity h ad a positiv e r elation ship w ith the diameter of hyph al pel let . Key words Phanerochaete chrysosporium, Avicel, L ignin perox idase, pH valu e. , pH 1 , (Phanerochaete chrysosp o . rium) [ 1] 2 [ 3] 2. 1 , . , , , Phanerochaete chrysosp orium PC26 PRL , . C , 30 # 2 , , . ( ) , 4 , . , . 1 ∃ 107 ∀ml- 1 , C CM C , 1995 P . Harvey . *