《蛋白质-核酸相互作用技术》相关实验技术-BioVyon Protein A for ChIP.pdfVIP

Analytical Biochemistry 412 (2011) 183–188 Contents lists available at ScienceDirect Analytical Biochemistry journal homepage: www.else /locate/yabio BioVyon Protein A, an alternative solid-phase affinity matrix for chromatin immunoprecipitation Igor Chernukhin a,1, Sung Yun Kang a,1, Sam Brown b, Svetlana Gretton a, Claudia Fabiola Mendez-Catala a, Dave Cowieson b, Elena Klenova a,⇑ a Department of Biological Sciences, University of Essex, Colchester, Essex CO4 3SQ, UK b Porvair Technology, A Division of Porvair Filtration Group, Wrexham LL13 9XS, UK a r t i c l e i n f o a b s t r a c t Article history: Chromatin immunoprecipitation (ChIP) is an important technique in the study of DNA/protein interac- Received 12 November 2010 tions. The ChIP procedure, however, has limitations in that it is lengthy, can be inconsistent, and is prone Received in revised form 25 January 2011 to nonspecific binding of DNA and proteins to the bead-based solid-phase matrices that are often used for Accepted 25 January 2011 the immunoprecipitation step. In this investigation, we examined the utility of a new matrix for ChIP Available online 1 February 2011 assays, BioVyon Protein A, a solid support based on porous polyethylene. In ChIP experiments carried out using two antibodies and seven DNA loci, the performance of BioVyon Protein A was significantly bet- Keywords: ter,