利用基因芯片技术阐述甘草酸协同麻黄碱的分子-天津中草药杂志社.PDF

第 33 卷 第 2 期 2010 年 4 月 - 85 - • 研 究 论 文 • 利用基因芯片技术阐述甘草酸协同麻黄碱的分子作用机制 石 倩，刘 赜，李若洁，程彬峰，侯媛媛，白 钢* 南开大学 药学院，天津 300071 摘 要 目的：利用基因表达谱芯片探索甘草酸协同麻黄碱对人支气管平滑肌细胞的协同作用机制。方法：采用 TNF-α 诱 导人支气管平滑肌细胞建立炎症模型，再分别用麻黄碱、麻黄碱联合甘草酸进行干预。提取细胞的 mRNA ，反转 录后与基因表达谱芯片进行杂交，经扫描分析筛选表达差异的基因。 结果：共筛选出显著表达差异基因 53 种，其 中33 种基因表达水平上调，20 种基因表达下调，经 Gene Go 软件分析这些基因在不同生物途径中存在协同作用。 结论：甘草酸在 G 蛋白偶联受体途径、细胞周期与凋亡以及离子通道等不同途径协同麻黄碱发挥作用。 关键词 麻黄碱；甘草酸；基因芯片；人支气管平滑肌 中图分类号：R969.2 R974.3 文献标志码：A 文章编号：1674–6376 （2010 ）02–0085–06 Analysis of synergic effect of glycyrrhizic acid and ephedrine on the human bronchial smooth muscle cells with cDNA microarray SHI Qian, LIU Ze, LI Ruo-jie, CHENG Bin-feng, HOU Yuan-yuan, BAI Gang* College of Pharmacy, Nankai University, Tianjin 300071, China Abstract Objective: To investigate the synergic effect of glycyrrhizic acid and ephedrine on the human bronchial smooth muscle cells with cDNA microarray method. Methods: After establishment of inflammation model induced by TNF-α, the human bronchial smooth muscle cells were divided into three groups randomly: the model group, ephedrine group, and glycyrrhizic acid plus ephedrine group. Total RNA of the subcutaneous tumors was extracted using Trizol reagents, and cDNA-labeled fluorescence was synthesized by reverse transcription method. Total DNA preparations from the cells were hybridized to the gene chips. Then the array was scanned and the signals were analyzed after hybridization. Results: Compared with ephedrine group, 33 genes were up-regulated by more than two folds and 20 genes were down-regulated in the HBAMC cells treated with glycyrrhizic acid plus ephedrine. The Gene