美洲大蠊药材HPLC指纹图谱研究 吴.pdfVIP

Advances in Analytical Chemistry 分析化学进展, 2015, 5(3), 39-48 Published Online August 2015 in Hans. /journal/aac /10.12677/aac.2015.53005 Fingerprint of Periplaneta americana by HPLC 1 1 2 2 1* Hongmei Wu , Ye Yang , Limei Liu , Ming Yang , Xiangpei Wang 1 Guiyang College of Traditional Chinese Medicine, Guiyang Guizhou 2 Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicines, Chengdu University of Traditional Chinese Medicine, Chengdu Sichuan * Email: wxp0123@126.com th th st Received: Jul. 28 , 2015; accepted: Aug. 14 , 2015; published: Aug. 21 , 2015 Copyright © 2015 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract Taking Diamonsil C18(1) column (250 mm × 4.6 mm, 5 μm), with acetonitrile-0.1% trifluoroacetic acid as mobile phase, detection wavelength 256 nm, we got a result that there were 22 common peaks in batches of Periplaneta Americana, the peaks similarity was more than 0.950, three com- mon peaks were identified, the similarity of Periplaneta fulginosa was 0.990 by comparing with the control fingerprint, but there were 18 common peaks. The method is repeatable and feasible and can be suitable for the quality control of Periplaneta americana. Keywords Periplaneta americana, Characteristic Chromatographic Profile, HPLC 美洲大蠊药材HPLC指纹图谱研究 1 1 2 2 1* 吴红梅 ，杨 烨 ，刘李梅，杨 明 ，王祥培 1贵阳中医学院，贵阳 2成都中医药大学中药材标准化教育部重点实验室，成都 * Email: wxp0123@1