碲化镉量子点作为探针研究核黄素与鲑鱼精DNA的相互作用CdTe.PDFVIP

2011 年第69 卷 化 学 学 报 Vol. 69, 2011 第16 期, 1951～1956 ACTA CHIMICA SINICA No. 16, 1951～1956 ·研究简报· 碲化镉量子点作为探针研究核黄素与鲑鱼精DNA 的相互作用 张渝阳*,a,c 李荧荧a 赵 琨b,c 赵 玲a 臧树良a,c (a 辽宁大学化学院 沈阳 110036) (b 沈阳药科大学药学院 沈阳 110016 ) (c 辽宁大学稀散元素研究所 沈阳 110036) 摘要 在水相中合成了巯基丙酸包覆的CdTe 量子点(CdTe QDs), 以CdTe QDs 作为探针, 在pH 7.25 Britton-Robinson (B-R)缓冲溶液中, 应用荧光光谱法、紫外吸收光谱法, 对核黄素(RF)与鲑鱼精DNA 作用方式进行了研究. RF 与DNA 作用时, 使荧光强度降低, 紫外吸收明显减色, 通过盐效应实验和DNA 热变性实验, Scatchard 方程等手段, 进一步研究 了RF 与DNA 的相互作用方式. 实验结果表明, RF 与DNA 作用方式可能为静电吸附和嵌插两种混合作用方式. 关键词 量子点; 核黄素; 鲑鱼精DNA; 荧光光谱; 嵌插作用 CdTe Quantum Dots as a Probe for Interaction between Salmon Sperm DNA and Riboflavin Zhang, Yuyang*,a,c Li, Yingyinga Zhao, Kunb,c Zhao, Linga Zang, Shulianga,c (a College of Chemistry, Liaoning University , Shenyang 110036) (b School of Pharmacy, Shengyang Pharmaceutical University, Shenyang 110016) (c Institute of Rare and Scattered Elements, Liaoning University , Shenyang 110036) Abstract The water-soluble CdTe quantum dots (QDs) were synthesized using mercaptopropionic acid as stabilizer. The interaction of riboflavin (RF) with salmon sperm DNA was investigated using UV and fluo- rescent spectra by employing CdTe QDs as a fluorescence probe in pH 7.25 Britton-Robinson (B-R) buffer solution. Decreased fluorescence and hypochromism of UV spectra were observed as RF interaction with DNA. The interaction of riboflavin with DNA has also been studied through