人类杀菌肽基因cDNA片段的克隆-生命科学研究.PDF

5 2 生命科学研究 V ol . 5 N o . 2 2 0 0 1 6 Lif e Science Research June 2001 a cDNA 1 2 2 1 2 3 1 杨　宇, 陈玉祥, 刘水平, 易伟峰, 陈铁骅, 文继舫, 胡维新 ( 1. , 410078; 2 . , 4 1007 8; 3. , 41007 8 : 采用 - 方法, 从慢性粒细胞白血病患者的外周血白细胞中扩增得到长度 为 1. 5 ¹ 的人类 RT P CR kb 杀菌肽cDN A , 并进一步得到长度 为600 bp 的该多肽的氨基端部分cDN A 扩增产物. 将上述两种长度的扩 增产物分别克隆至 - 载体中, 为进一步开展该重组抗菌肽的表达和活性研究奠定了基础. pU Cm T : 杀菌肽; 慢性粒细胞白血病; 分子克隆 : Q 523 . 1; Q 754: A : 1007-7847 ( 200 1 02-0133-04 Cloning of the cDNA Fragments of Human / - Bactericidal Permeability increasing Protein 1 2 2 1 YA N G Yu , CHEN Yu -xiang , L IU Shui-ping , Y I W ei-feng , 2 3 1 CHEN T ie-hu a , WEN Ji-f ang , HU Wei-x in ( 1. M olecular Biology Research Center, X iangya Med ical College, Central S outh Univ ersity , Changsha 4 1007 8, H unan, ; 2. , , 4 1007 8, , China Dep artment of p harmaceutics X iangya M edical College Central South University Changsha H unan China; 3. Dep artment of p athematology , X iangya Med ical College, Central South Univ ersity , Changsha 410078, H unan, China Abstract: By using RT -PCR m et hod , the