β-榄香烯对肺腺癌细胞株A549体外放射增敏作用实验分析.pdfVIP

influenceof to aimstoevaluatethe 13-elemene Objective：Thisstudy to thatif humancancerA549cellson andinvestigate lung radiosensitivity thereiscorrelationbetween andthe levelof radiosensitivityexpression KU70andKUS0 gene． A549cellsastheresearch was Method：Taking object，13-elemene incommon Dalian Medicine werecultured producedby Jingang Company．Cells culture RPMI．1640culturemedium．Thetest were tothe groups exposed concentritionsof 1 mediumatdifferent 0ug／ml、20ug／ml、 p—elemene(0ug／ml control hadnop。 group 40ug／ml、80ug／ml、160ug／ml、320ug／m1)．The were toradiationatdifferent inculturemedium．Then elemene they exposed 1 indexesweredetected doseof 0Gy)．All X—ray(0Gy，2Gy，4Gy，6Gy，8Gy A549cellsculture rateoncell after finished．Inhibitingproliferation concentritionswasevaluatedwitha ofMTT todifferent way exposed calculateIC50of ofA549cellswas assay．，then 24h．Radiosensitivity the