用酯酶同功酶分析白僵菌的林间扩散能力-中国生物防治学报.PDF

( ) 21 4 238 - 241 　　　　　　中国生物防治 　Chinese Journal of Biological Control 　　　　　　2005 年 11 月 用酯酶同功酶分析白僵菌的林间扩散能力 1 ,2 ,3 2 2 3 1 胡加付 , 缪 　凯 , 柴守权 , 骆有庆 , 李增智 ( 1 安徽农业大学 , 合肥 　230036 ; 2 国家林业局森防总站 , 沈阳　110034 ; 3 北京林业大学 , 北京　100083) 摘要 : 在林间人工放菌和挂笼防治光肩星天牛试验的基础上 ,分离受感染的光肩星天牛白僵菌 菌株 ,对分离的菌株用电泳法进行酯酶同工酶酶谱分析 ,用聚类分析法对不同分离株进行分类 , 并进一步分析白僵菌在林间的扩散和交叉感染作用 。结果发现 :在 50m 范围内, 白僵菌可以有效 地感染目标害虫 ,显示出较强的林间扩散能力和病害流行潜力 。 关 　键 　词 : 白僵菌 ; 光肩星天牛; 酯酶同工酶 ; 聚类分析 ; 流行潜力 ( ) 中图分类号 : S47612 ; Q556 　　文献标识码 : A 　　文章编号 : 2005 Evaluation on the Spread of Beauveria bassiana in Forest with Esterase Isoenzymes HU Jiafu , MIAO Kai , CHAI Shouquan , LUO Youqing , Li Zengzhi (Anhui Agricultural University , Hefei 230036 , China) Abstract : After nonwoven fabric bands impregnated with B eauveria strains ND and MT were released for biological control against A nop lop hora glabripennis with cages in willow forest , the affected adults of A . glabrip ennis were collected and cultured in lab for strain reisolation . The esterase isoenzymes of different isolated strains were analyzed by gel electrophoresis. The isolated strains were classified through clustering analysis for further analyzing the spreading and crossinfection ability of B eauveria spores. The results indi cated that A . glabrip ennis could be effectively infected by B eauveria within a radius of 50 m. And B eauveria spores showed spread and epidemic potential . Key words : B eauveria bassiana ; A nop lop hora glabrip ennis ; esterase isozyme ; clustering analysis ; epi demic potential 虫生真菌作为生物杀虫剂的一种 ,其丰富的种质资源显示了潜在的应用前景 ,尤其是其流行