电喷雾离子迁移谱快速检测次乌头碱的研究-现代仪器与医疗.PDF

《现代仪器与医疗》 2018年  第24卷   第3期 检验检测 电喷雾离子迁移谱快速检测次乌头碱的研究 1 1 1 1 1 2 2 孔景临 　刘卫卫 　秦墨林 　李宝强 　丁俊杰 　陈创 　李海洋 1.防化研究院，北京　102205；2. 中国科学院大连化学物理研究所，辽宁大连　116023 [摘　要]　利用自行搭建的电喷雾离子迁移谱装置对中草药中常见的剧毒成分次乌头碱进行检测研究。实验表 明离子门的开门时间对检测灵敏度影响较大，通过参数优化，自制的电喷雾离子迁移谱平台对次乌头碱的检测 灵敏度可达800ng/mL，检测时间在1min 之内。此外，本实验用普通滤纸代替喷雾针，以纸喷雾方式实现了对 次乌头碱的检测。 [关键词]　电喷雾；纸喷雾；离子迁移谱；次乌头碱 中图分类号：R331　文献标识码：A　文章编号：2095-5200(2018)03-099-04 DOI：10.11876/mimt201803041 Studies on Fast Detection of Hypaconitine by Electrospray Ionization-Ion Mobility Spectrometry　 1 1 1 1 1 2 2 Kong Jinglin , Liu Weiwei , Qin Molin , Li Baoqiang , Ding Junjie , Cheng Chuang , Li Haiyang . 1.Institute of Chemcial Defense, Beijng 102205 2. Dalian Institute of Chemical Physics Chinese Academy of Sciences, ； Dalian 116023,China [Abstract]　An electrospray ionization-ion mobility spectrometer (ESI-IMS) was built up and was used to detect hypaconitine, which was a kind of highly toxic component in some traditional Chinese medicines. The effect of the parameter such as the opening time of ion gate was studied and optimized ．The results showed that the limit of detection of this instrument for hypaconitine could reach 800 ng/mL within 1min ．Spray needle was substituted by ordinary filter paper and a paper spray ionization-ion mobility spectrometer (PSI-IMS) was implemented in this report. Data shown herein indicate that paper spray ambient ionization can be readily used as a rapid and robust method for in situ direct analysis of hypaconitine. [Key words] Electrospray Paperspray Ion mobility spectrometer Hypaconitine 　 ； ； ； 离子迁移谱（Ion Mobility Spectrometry ，IMS ）技术 ESI-IMS 大大拓宽了传统IMS 技术检测对象范围，为 是20 世纪中期发展起来的一种微