枯草芽孢杆菌作为新的益生菌演讲话稿.pptVIP

New Bacillus subtilis Strains as Promising Probiotics FRONTIERS IN IMMUNOLOGY CONTENTS 01 02 03 04 INTRODUCTION MATERIALS AND METHODS RESULTS AND DISCUSSION CONCLUSION INTRODUCTION Probiotics: live microorganisms positive effect on diverse functions of an organism prevent the invasion of various pathogens Experiments with mice proved that B. subtilis spores were able to germinate, and the cells could proliferate and form the spores again in intestines of animals Autochthonous lactic acid bacteria of the genera Lactobacillus and Bifidobacterium are usually used as probiotics; gram positive spore-forming bacteria of thegenus Bacillus are used as probiotics as well  Studies have revealed that in addition to the resistance of the probiotic Bacillus strains to bile acids, they were capable of immune stimulation in case of gastrointestinal disorders. Ability to synthesize antibiotics, bacteriocins, cyclic lipopeptides, and lytic enzymes with antimicrobial activity provides probiotic activity for bacteria of the genus Bacillus . Being probiotics, the strains of B. subtilis and B. coagulans were shown to have a growth-stimulating and prophylactic effect on broiler chickens 1 INTRODUCTION 标题 The goal of the present work was to characterize the properties of these new strains and to assess their potential use as probiotics. 01 INTRODUCTION MATERIALS AND METHODS The subjects of the study were B. subtilis strains GM2 and GM5 with high antimicrobial activity (Mardanova et al., 2017). Opportunistic coliform bacteria, Salmonella enterica serotype Typhimurium ATCC14028s, Klebsiella oxytoca, and Escherichia coli, as well as micromycetes Fusarium avenaceum, F. oxysporum, F. redolens, and F. solani, were used as test cultures.  02 MATERIALS AND METHODS Nutrient media and cultivation conditions. The following media were used for cultivation. These were:(1) LB (Luria–Bertoni) medium (g/L): tryptone, 10.0; yeast extract, 5.0; NaCl, 5.0; (2) LA medium(g/L): tryptone, 10.0