PEG沉淀法-病毒提纯方法.docx

1. Viruses such as baculovirus can be concentrated by polyethylene glycol precipitation.2. Adjust the pH of the virus-containing supernatant to 7.2-7.5. Polyethylene glycol (PEG, MW 6,000) is added to a final concentration of 8% (w/v).3. Stir at 4℃for 2 hours.4. Centrifuge at 10,000 x g for 2 hours.5. Resuspend the pellet in a small volume of appropriate buffer ( e.g., RNase-free ddH2 for virus RNA prep). 沉淀法-病毒提纯方法主要是从稀的悬浮液中浓缩病毒，有中性盐沉淀法、聚乙二醇(PEG)沉淀法、有机溶剂沉淀 法、等电点沉淀法、皂土法和鱼精蛋白沉淀法等。　1.中性盐沉淀法：病毒一般在45%以上饱和度的硫酸铵溶液中沉淀，且保持其感染性。在含有病毒的组织培养液中加入等体积的饱和硫酸铵，可以很容易地沉淀某些病毒如狂犬病毒、鸡新城疫病毒等。　　2.聚乙二醇沉淀法：聚乙二醇(