肉桂醛对恶性黑素瘤A375细胞增殖和表达VEGF的.doc

PAGE 肉桂醛对恶性黑素瘤A375细胞增殖和表达VEGF的影响 周 凌，鲁元刚，杨桂红，杨 涛，伍津津 （400042 重庆，第三军医大学大坪医院野战外科研究所皮肤科） [摘要] 目的 研究肉桂醛对体外培养的人恶性黑素瘤细胞株A375增殖和分泌血管内皮细胞生长因子（vascular endothelial growth factor， VEGF）的影响。方法 A375细胞在体外培养扩增后，在培养基中分别加入10、20、40 μmol/L肉桂醛，分别在培养24、48、72 h MTT法检测细胞活性。同时收集各个浓度肉桂醛处理不同时相的培养液样本，双抗体夹心ELISA法测定其中VEGF分泌量。结果 10 μmol/L肉桂醛作用24 h即能抑制A375细胞增殖，10、20、40 μmol/L肉桂醛间的抑制强度具有统计学意义（P＜0.01）。40 μmol/L的肉桂醛作用72 h后细胞增殖抑制率最高，达(88.91±4.5)%。随肉桂醛作用时间延长，A375细胞分泌VEGF的量逐渐减少，72 h后各浓度间的抑制作用具有统计学意义（P＜0.01）。40 μmol/L肉桂醛作用72 h后A375细胞分泌的VEGF含量最低，为9.55 pg/ml。结论 肉桂醛能抑制人黑素瘤细胞增殖和VEGF的分泌，其抑制强度与药物浓度和作用时间成正比。 [关键词] 肉桂醛；黑素瘤细胞；血管内皮细胞生长因子；增殖 [中图法分类号] [文献标志码] A Effect of cinnamic aldehyde on proliferation and VEGF secretion in human melanoma A375 cells Zhou Ling， Lu Yuangang，Yang Guihong，Yang Tao，Wu Jinjin （Department of Dermatology, Daping Hospital, Third Military Medical University, Chongqing, 400042, China） [Abstract] Objective To investigate the effect of cinnamic aldehyde on proliferation and level of vascular endothelial growth factor (VEGF) secreted in A375 cells. Methods A375 cells were co-cultured with cinnamic aldehyde of the concentration of 10, 20 and 40μM for 24, 48 and 72 hours. The proliferation of A375 cells was tested by MTT methods. The VEGF protein expression level was detected by ELISA. Results Cinnamic aldeyde of 10μM inhibited A375 cells proliferation after 24 hours . The concentration of cinnamic aldeyde affected the cell proliferation significantly(P＜0.01). The proliferation of A375 cells was most remarkably inhibited after co-culture of 40μM cinnamic aldeyde for 72 hours. The highest inhibition rate is 88.91±4.5%. The VEGF level was lower with the longer co-culture time. The concentration of cinnamic aldeyde affected the VEGF secretion significantly (P＜0.01) in 72 hours. The lowest VEGF level of A375 cells was 9.55pg/ ml after co-culture of 40μM cinnamic aldeyde for 72 hours. Conclusions Cinnamic aldeyde inhibit the proliferation an