百合鳞茎发育过程中鳞片超微结构的变化.PDFVIP

园 艺 学 报 2010 ，37 （2 ）：247 –255 Acta Horticulturae Sinica 百合鳞茎发育过程中鳞片超微结构的变化 * 吴沙沙，吕英民 ，张启翔 (北京林业大学园林学院，国家花卉工程技术研究中心，北京 100083) 摘 要：以东方百合‘索邦’（Lilium oriental hybrids ‘Sorbonne’）为试材，利用透射电镜技术，对百 合鳞茎发育过程中外、中层鳞片的超微结构进行了系统观察和分析。结果表明：栽种期鳞片细胞中淀粉粒、 蛋白质、脂滴数量最多，体积最大，鳞茎起着“源”的功能。随着植株的生长，靠近细胞壁和淀粉粒分布的 大量的线粒体证明在此过程中鳞片细胞中以分解代谢为主，淀粉粒、蛋白质和脂滴数量呈下降趋势，至盛花 期时达到最低。之后鳞茎细胞内开始大量贮藏同化产物，淀粉粒、蛋白质和脂滴的数量明显增多，在花后期 鳞茎转变以发挥 “库”的功能，收球期淀粉粒和蛋白质再次充满整个细胞。同时观察到外层、中层鳞片细 胞都存在大量成束出现的胞间连丝，且在盛花期时胞间连丝的通道中有成列的小囊泡，证明了百合鳞片在 “库”—“源”功能转化的过程中主要是以共质体途径进行物质的交换和运输。 关键词：百合；发育时期；库源关系；鳞片；超微结构 中图分类号：S 682.2 文献标识码：A 文章编号：0513-353X(2010)02-0247-09 The Ultra-structure Changes of Scales During the Development of Lily Bulb * WU Sha-sha ，Lü Ying-min ，and ZHANG Qi-xiang (College of Landscape Architecture，Beijing Forestry University ，China National Floriculture Engineering Research Centre， Beijing 100083，China) Abstract ：Ultra-structure of outer and middle scales of bulbs of Lilium oriental hybrids cultivar ‘Sorbonne’ were observed and analyzed systematically by using the transmission electron microscope (TEM) during the bulb development. The results indicated that there were a lot of big starch granules, proteins and lipid droplets in the scale cells at the planting stage. The bulbs played the role of “source” at this stage. With the growth of lily plant, a great number of mitochondria appeared near the cell wall and around the starch grains. This indicated that catabolism was the predominant processes in scale cells. The number of starch granules, proteins and lipid droplets decreased and reached the minimum at the blooming stage. After blooming, the amount of starch granules, proteins and lipid droplets increased significantly, the bulb converted to play the role of “