固相微萃取.doc

PAGE 6 食品安全质量检测学报 第8卷 第3期 林 霞, 等: 固相微萃取-气质联用法测定2种延命草属植物的挥发性物质 PAGE 5 第8卷 第3期 食品安全质量检测学报 Vol. 8 No. 3 2017年3月 Journal of Food Safety and Quality Mar. , 2017 基金项目: 福建农林大学教学类项目(0137e9) Fund: Supported by Teaching Projects of FAFU (0137e9) *通讯作者: 林霞, 硕士, 讲师, 主要研究方向为农业与生物技术。E-mail: *Corresponding author: LIN Xia, Master, Lecturer, Jinshan College of Fujian Agriculture and Forestry University, Fuzhou 350000, China. E-mail: 固相微萃取-气质联用法测定2种延命草属植物的挥发性物质 林 霞* (福建农林大学金山学院, 福州 350000) 摘 要: 目的 考察2种形态上类似的延命草属植物在挥发性物质的组成与相对含量上的不同。方法 以Plectranthus tomentosa(碰碰香)和Plectranthus sp.(未定名)为研究对象, 采用固相微萃取-气相色谱-质谱联用法(solid-phase microextraction combined with gas chromatography-mass spectrometry, SPME-GC-MS)分析叶片的挥发性成分, 再使用峰面积归一化法确定成分的相对含量。结果 通过NIST谱库检索, 从Plectranthus tomentosa中初步鉴定出高匹配成分39个, 占所有检出峰总相对含量的81.78%; 从Plectranthus sp.中鉴定高匹配成分41个, 占所有检出峰总相对含量的80.62%。挥发性物质中包含3个高含量成分, 分别为柠檬烯、乙酸龙脑酯、石竹烯。这2种植物包含25种共有成分, 同时其挥发性物质也存在差异, Plectranthus tomentosa中包含13种特异性成分, 而Plectranthus sp.中包含16种。结论 本研究为2种延命草属植物的进一步开发和利用提供参考。 关键词: 延命草属; 挥发性气味; 固相微萃取-气相色谱-质谱联用法 Determination of volatile components from 2 Plectranthus genera plants by solid-phase microextraction combined with gas chromatography-mass spectrometry LIN Xia* (Jinshan College of Fujian Agriculture and Forestry University, Fuzhou 350002, China) ABSTRACT: Objective To investigate the differences of the compositions and relative content of volatile substances between two similar Plectranthus genera plants. Methods Plectranthus tomentosa and Plectranthus sp. were taken as the samples, solid-phase microextraction combined with gas chromatography-mass spectrometry (SPME-GC-MS) was used to analyze the volatile components, and the peak area normalization method was used to determine the relative content of the components. Results Using NIST spectrum library retrieval, 39 high matching components were identified from Plectranthus tomentosa, with the relative content of 81.78%, and 41 high matchin